Department+of+Chemical+and+Biomolecular+Engineering
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Distinguished Professor Lee Sang Yeop Appointed as Fellow of the American Institute of Chemical Engineers
Professor Lee Sang Yeop (Dean of the Department of Biological Sciences) has become the first Korea Scientist to be appointed as the Fellow of the American Institute of Chemical Engineers.
The American Institute of Chemical Engineers was founded in 1908 and boasts a 100 year history. It is composed of 43,000 members over 90 countries and is the largest international Academic Institute in the field of Chemical Engineering.
The Institute appoints Fellows after a rigorous procedure of recommendation and evaluation and Professor Lee is the first Korean to become a Fellow.
Professor Lee’s expertise is the field of Metabolic Engineering and successfully applied the system design method and optimization strategy of chemical engineering to biological systems thereby developing numerous core technologies for the biology based chemical industries.
Professor Lee is the founder of the System Metabolic Engineering and enabled the medical application of microorganisms by manipulating the metabolic pathways on a systems level in addition to making great progress in synthesizing various oil originated chemical materials using biology based, environmentally friends methods.
Professor Lee received the Marvin J. Johnson Award, Charles Thom Award, and has been appointed by the first Chairman of the Biotech Global Agenda Counsel of the World Economic Forum.
2012.09.22 View 8295 -
Distinguished Professor Sang-Yeop Lee gave keynote speech in '2011 China Bio-Refinery Summit'
Distinguished Professor Sang-Yeop Lee gave keynote speech in ‘2011 China Bio-Refinery Summit’ held in Chang’an, Beijing
Professor Lee gave a lecture on the vitalization strategy of ‘Bio-Refinery’, which is ‘A bio-based chemical industry to replace fossil fuel-based petro chemistry.
Professor Lee, insisted that for the successful construction of ‘Bio-Refinery’, there should be innovation in all value chain of biomass; biomass producer, bio-refinery business, consumer, government, etc. ▲Securement and distribution of Biomass ▲Development of strain and process for fermentation separation to effectively change biomass into chemical substance and fuel ▲Optimization of transportation and marketing.
During this summit, high-ranking government officials in politics and economics, executives of multicultural and Chinese business participated. From Korea, Do-Young Seung of Manager of technology research of GS and Hang-Deok Roh of laboratory chief of SK Chemical participated as panelist.
World Economy Forum, the gathering of leaders and experts in politics, economics, and policy created a ‘Global Agenda Council’ to find solutions on the issue of ‘sustainable growth of environment of the Earth and humanity’. Professor Lee is the chairperson of ‘Emerging Technologies Global Agenda Council (GAC)’ of Word Economy Forum.
Professor Lee, founder of ‘Systems Metabolic Engineering’, has made remarkable achievements world-wide, including a technology that manipulates metabolic circuit of microorganisms to purify various crude-originated chemical substances into environmentally friendly substances.
Currently, he is working on Systems biology research business in Ministry of Education, Science and Technology, Global Frontier Biomass business, Global Frontier Intelligent Bio-system construction and composition, to make progress in metabolic engineering which is essential for the bio-chemical industry.
2012.03.06 View 10837
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The Hindu, "Use of microalgae helps in controlling pollution," December 8, 2011
The Hindu, an Indian newspaper, reported on December 8, 2011 a research work by Professor Ji-Won Yang from the Department of Chemical and Biomolecular Engineering. For the news article, please go to the link at http://www.thehindu.com/sci-tech/energy-and-environment/article2695634.ece?homepage=true.
The Hindu, December 8, 2011
Use of microalgae helps in controlling pollution
By N. Gopal Raj
2011.12.12 View 9059
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Professor Lee Sang Yeop Nominated the Chairman of Emerging Technologies Global Agenda Council of the World Economic Forum
Professor Lee Sang Yeop, Dean of College Life Science & Bioengineering, was appointed as the chairman of the Emerging Technologies Global Agenda Council of the World Economic Forum.
He will be in office till the 31st of August 2012, exactly 1 year from the date of his appointment.
The World Economic Forum (WEF) is a ‘think tank’ consisting of world leaders in various fields like economics, politics, and policies and has created the ‘Global Agenda Council’ to solve the problems mankind faces in achieving environmentally sustainable growth and suggest a collective vision and strategy.
The committee to be chaired by Professor Lee (Emerging Technologies Global Agenda Council) will discuss the direction in which the fields of biological engineering, nanotechnology, and IT (information technology) should develop and discuss the possible impact these fields will have on the society.
Professor Lee commented that, “I am extremely happy to be appointed as the chair of the Emerging Technologies Global Agenda Council at the World Economic Forum which is a gathering of world class leaders” and that “it is a great opportunity to spread Korea’s success and lessons in the advancement of science and technology.”
Professor Lee is the creator of the field of system metabolism engineering and is making great strides in manipulating the microorganism’s metabolic pathways on a systems scale to make changing chemicals derived from oil into eco-friendly and bio-based products.
2011.09.20 View 8973 -
College of Cultural Science selects 'Best English Paper' Recipients
KAIST’s College of Cultural Science (Dean Kim Dong Won) announced the winners of ‘2010 Fall Semester Best English Paper Award’: Excellence Award went to Kwak Ah Young (department of Biology), Seong Du Hyun (undeclared major), Ahn Da In (Biological Chemical Engineering), and nine other students received the Participation Award.
Ahn Da In discussed ‘Joyce and Chopin on use of epiphany’, Seong Du Hyun discussed ‘On Kant’s Groundwork for the metaphysics of Morals, its Achievements, and Implications’, and Kwak Ah Young discussed ‘Fact Pattern of Environmental Law’.
The ‘Best English Paper’ Award has been awarded to undergraduates since 2009 Fall semester in the Humanities and Social Science Department’s efforts to increase creativity and English writing skills of students attending KAIST.
For the 2010 fall semester, each professor in charge went through 1 to 2 papers (among 610 papers admitted across human science subjects) and recommended 29 papers to be discussed with great scrutiny. The evaluation took place throughout January and the 3 papers discussing English Literature, Scientific Philosophy, and Environmental Law were awarded the Excellence Award, and 9 other papers were awarded the Participation Award.
Dean of Cultural Science College Kim Dong Won commented, ‘I am very encouraged by the level of papers and is becoming a very large art of the College of Cultural Science’.
The chairman of the screening committee, Professor Kim Eun Kyung also commented, ‘the best paper award is helping students better their English levels’ and that ‘in order to form and encourage a sound and structured English paper writing environment, a anti-plagiarism program will be implemented amongst implementing other judging standards’.
2011.02.21 View 10206
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Explanation for the polymerized nucleic acid enzyme's abnormal activation found
KAIST’s Professor Park Hyun Kyu of the Department of Bio Chemical Engineering revealed on the 23rd of December 2010 that his team had successfully developed the technology that uses the metal ions to control the abnormal activation of nucleic acids’ enzymes and using this, created a logic gate, which is a core technology in the field of future bio electrons.
The polymerized nucleic acid enzyme works to increase the synthesis of DNA and kicks into action only when the target DNA and primers form complimentary pairs (A and T, C and G).
Professor Park broke the common conception and found that it is possible for none complimentary pairs like T-T and C-C to initiate the activation of the enzyme and thus increase the nucleic acid production, given that there are certain metal ions present.
What Professor Park realized is that the enzymes mistake the uncomplimentary T-T and C-C pairs (with stabilized structures due to the bonding with mercury and silver ions) as being complimentary base pairs. Professor Park described this phenomenon as the “illusionary polymerase activity.”
The research team developed a logic gate based on the “illusionary polymerase activity’ phenomenon.” The logic gate paves the way to the development of future bio electron needed for bio computers and high performance memories.
Professor Park commented, “The research is an advancement of the previous research carried on about metal ions and nucleic acid synthesis. Our research is the first attempt at merging the concepts of the two previously separately carried out researches and can be adapted for testing presence of metal ions and development of a new single nucleotide polymorphic gene analysis technology.”
Professor Park added that, “Our research is a great stride in the field of nano scale electron element research as the results made possible the formation of accurate logic gates through relatively cost efficient and simple system designs.”
On a side note, the research was funded by Korea Research Foundation (Chairman: Park Chan Mo) and was selected as the cover paper for the December issue of ‘Angewandte Chemie International Edition’.
2011.01.18 View 9934 -
Nanowerk Spotlight: Bacteria as environmentally friendly nanoparticle factories, Sep. 24, 2010
The Nanowerk.com is a leading portal site for nanotechnology and nanosciences, which runs a daily news section called “Spotlight.” On September 24, 2010, the Spotlight published an article on the latest developments of the research by a KAIST team headed by Distinguished Professor Sang-Yup Lee of the Chemical and Bimolecular Engineering Department. For the article, please click the link below:
Nanowerk Spotlight: Bacteria as environmentally friendly nanoparticle factories, Sep. 24, 2010
By Michael Berger.
http://www.nanowerk.com/spotlight/spotid=18188.php
2010.09.25 View 9959
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KAIST was invited to the World Economic Forum's fourth "Summer Davos."
KAIST attended the World Economic Forum’s “Summer Davos Forum” held from September 13 to 15 in Tianjin, China. The Summer Davos Forum hosted various sessions and meetings with international dignitaries from governments, business and public organizations, and academia on the main theme of “Driving Growth through Sustainability.”
On September 14, four subjects including “Electric Vehicles,” “Humanoid Robotics,” “Next Generation of Biomaterials,” and “New Developments in Neuroengineering” were presented by KAIST, followed by discussions with forum participants.
Professor Jae-Seung Jeong of the Bio and Brain Engineering Department, Sang-Yup Lee of the Chemical and Biomolecular Engineering Department, Joon-Ho Oh of the Mechanical Engineering Department, and President Nam-Pyo Suh participated in the forum as presenters of the topic. Of these speakers, Professors Jae-Seung Jeong and Sang-Yup Lee were nominated by the World Economic Forum (WEF) as members of the “Young Global Leader” and “Global Agenda Council on Emerging Technologies,” respectively.
President Suh was also invited to the CEO Insight Group and delivered an opening speech on OLEV (Online Electric Vehicle) and the Mobile Harbor. President Suh plans to sign an MOU for research cooperation with Jong-Hoo Kim of Bell Lab and Shirley Jackson of the Rensselaer Polytechnic Institute in the near future, respectively.
Since 2007, the WEF, in charge of the world’s largest international conference called “Davos Forum” has hosted a “Summer Davos Forum,” also called as the “Annual Meeting of New Champions.” The Summer Davos Forum consists of nations, rising global companies, next generation of global leaders, and cities or nations that lead technological innovations. Unlike the annual Davos Forum held in January, the “Annual Meeting of New Champions” is held in September of each year in Tianjin and Dalian, China.
Since 2009, the WEF has added a special session called IdeasLab in the Davos and Summer Davos Forums. Through IdeasLab, prominent universities from all over the world, research organizations, venture businesses, NGOs, and NPOs are invited to exchange and discuss innovative and creative ideas that can contribute to the development of mankind. Until now, universities including INSEAD, EPFL-ETH, MIT, Oxford, Yale, Harvard, Rensselaer Polytechnic Institute, Tsinghua University, and Keio University have been invited to the IdeasLab. KAIST is the first Korean university to attend this session.
2010.09.17 View 17442 -
Bioengineers develop a new strategy for accurate prediction of cellular metabolic fluxes
A team of pioneering South Korean scientists has developed a new strategy for accurately predicting cellular metabolic fluxes under various genotypic and environmental conditions. This groundbreaking research is published in the journal Proceedings of the National Academy of Sciences of the USA (PNAS) on August 2, 2010.
To understand cellular metabolism and predict its metabolic capability at systems-level, systems biological analysis by modeling and simulation of metabolic network plays an important role. The team from the Korea Advanced Institute of Science and Technology (KAIST), led by Distinguished Professor Sang Yup Lee, focused their research on the development of a new strategy for more accurate prediction of cellular metabolism.
“For strain improvement, biologists have made every effort to understand the global picture of biological systems and investigate the changes of all metabolic fluxes of the system under changing genotypic and environmental conditions,” said Lee. The accumulation of omics data, including genome, transcriptome, proteome, metabolome, and fluxome, provides an opportunity to understand the cellular physiology and metabolic characteristics at systems-level. With the availability of the fully annotated genome sequence, the genome-scale in silico (means “performed on computer or via computer simulation.”) metabolic models for a number of organisms have been successfully developed to improve our understanding on these biological systems. With these advances, the development of new simulation methods to analyze and integrate systematically large amounts of biological data and predict cellular metabolic capability for systems biological analysis is important.
Information used to reconstruct the genome-scale in silico cell is not yet complete, which can make the simulation results different from the physiological performances of the real cell. Thus, additional information and procedures, such as providing additional constraints (constraint: a term to exclude incorrect metabolic fluxes by restricting the solution space of in silico cell) to the model, are often incorporated to improve the accuracy of the in silico cell.
By employing information generated from the genome sequence and annotation, the KAIST team developed a new set of constraints, called Grouping Reaction (GR) constraints, to accurately predict metabolic fluxes. Based on the genomic information, functionally related reactions were organized into different groups. These groups were considered for the generation of GR constraints, as condition- and objective function- independent constraints. Since the method developed in this study does not require complex information but only the genome sequence and annotation, this strategy can be applied to any organism with a completely annotated genome sequence.
“As we become increasingly concerned with environmental problems and the limits of fossil resources, bio-based production of chemicals from renewable biomass has been receiving great attention. Systems biological analysis by modeling and simulation of biological systems, to understand cellular metabolism and identify the targets for the strain improvement, has provided a new paradigm for developing successful bioprocesses,” concluded Lee. This new strategy for predicting cellular metabolism is expected to contribute to more accurate determination of cellular metabolic characteristics, and consequently to the development of metabolic engineering strategies for the efficient production of important industrial products and identification of new drug targets in pathogens.”
2010.08.05 View 12294 -
Native-like Spider Silk Produced in Metabolically Engineered Bacterium
Microscopic picture of 285 kilodalton recombinant spider silk fiber
Researchers have long envied spiders’ ability to manufacture silk that is light-weighted while as strong and tough as steel or Kevlar. Indeed, finer than human hair, five times stronger by weight than steel, and three times tougher than the top quality man-made fiber Kevlar, spider dragline silk is an ideal material for numerous applications. Suggested industrial applications have ranged from parachute cords and protective clothing to composite materials in aircrafts. Also, many biomedical applications are envisioned due to its biocompatibility and biodegradability.
Unfortunately, natural dragline silk cannot be conveniently obtained by farming spiders because they are highly territorial and aggressive. To develop a more sustainable process, can scientists mass-produce artificial silk while maintaining the amazing properties of native silk? That is something Sang Yup Lee at the Korea Advanced Institute of Science and Technology (KAIST) in Daejeon, the Republic of Korea, and his collaborators, Professor Young Hwan Park at Seoul National University and Professor David Kaplan at Tufts University, wanted to figure out. Their method is very similar to what spiders essentially do: first, expression of recombinant silk proteins; second, making the soluble silk proteins into water-insoluble fibers through spinning.
For the successful expression of high molecular weight spider silk protein, Professor Lee and his colleagues pieced together the silk gene from chemically synthesized oligonucleotides, and then inserted it into the expression host (in this case, an industrially safe bacterium Escherichia coli which is normally found in our gut). Initially, the bacterium refused to the challenging task of producing high molecular weight spider silk protein due to the unique characteristics of the protein, such as extremely large size, repetitive nature of the protein structure, and biased abundance of a particular amino acid glycine. “To make E. coli synthesize this ultra high molecular weight (as big as 285 kilodalton) spider silk protein having highly repetitive amino acid sequence, we helped E. coli overcome the difficulties by systems metabolic engineering,” says Sang Yup Lee, Distinguished Professor of KAIST, who led this project. His team boosted the pool of glycyl-tRNA, the major building block of spider silk protein synthesis. “We could obtain appreciable expression of the 285 kilodalton spider silk protein, which is the largest recombinant silk protein ever produced in E. coli. That was really incredible.” says Dr. Xia.
But this was only step one. The KAIST team performed high-cell-density cultures for mass production of the recombinant spider silk protein. Then, the team developed a simple, easy to scale-up purification process for the recombinant spider silk protein. The purified spider silk protein could be spun into beautiful silk fiber. To study the mechanical properties of the artificial spider silk, the researchers determined tenacity, elongation, and Young’s modulus, the three critical mechanical parameters that represent a fiber’s strength, extensibility, and stiffness. Importantly, the artificial fiber displayed the tenacity, elongation, and Young’s modulus of 508 MPa, 15%, and 21 GPa, respectively, which are comparable to those of the native spider silk.
“We have offered an overall platform for mass production of native-like spider dragline silk. This platform would enable us to have broader industrial and biomedical applications for spider silk. Moreover, many other silk-like biomaterials such as elastin, collagen, byssus, resilin, and other repetitive proteins have similar features to spider silk protein. Thus, our platform should also be useful for their efficient bio-based production and applications,” concludes Professor Lee.
This work is published on July 26 in the Proceedings of the National Academy of Sciences (PNAS) online.
2010.07.28 View 16687 -
The 8th International Conference on Metabolic Engineering was held on June 13-18, 2010 in Jeju Island, South Korea.
From left to right, top row: Distinguished Professor and the conference chair Sang Yup Lee, Sang-Hyup Kim - Secretary to the President of Korea, Dr. Jay Keasling, Dr. Greg Stephanopoulos. Left to right, bottom row: Dr. William Provine, Dr. Terry Papoutsakis, Dr, Jens Nielsen, Dr. Lars Nielsen.
The importance of industrial biotechnology that produces chemicals and materials from renewable biomass is increasing due to climate change and the dearth of natural resources. Industrial biotechnology refers to a technology that allows sustainable bio-based production of chemicals and materials that could enrich human"s lives using microorganisms. This is where metabolic engineering comes into play for successful application of microorganisms, in which they are engineered in our intended way for improved production capability.
The 8th International Conference on Metabolic Engineering, the longest running conference of its kind, was held on June 13-18, 2010 at the International Convention Center in Jeju Island, South Korea. Distinguished Professor Sang Yup Lee of KAIST, Dean of College of Life Science and Bioengineering and Co-Director of Institute for the BioCentury, chaired the conference with the main theme of "metabolic engineering for green growth."
With 300 delegates selected by the committee, papers on production of biofuels, chemicals, biopolymers, and pharmaceutics and the development of fundamental metabolic engineering techniques were presented at the conference along with examples of successful commercialization of products developed by several global companies.
Sang Hyup Kim, Secretary to the President of Korea, gave an opening plenary lecture entitled "Korean green growth initiative," to inform experts from around the globe of the leadership on green growth in Korea. Young Hoon Park, President of Korea Research Institute of Bioscience and Biotechnology (KRIBB, Korea) delivered his congratulatory address.
Sang Hyup Kim said, "Hosting an international conference in Korea on metabolic engineering, which forms a core technology necessary for the development of environmentally friendly processes for producing chemicals and biofuels from renewable biomass, is very meaningful as green growth is a big issue around the globe. This is a great chance to show the excellence of Korea"s green growth associated technology to experts in metabolic engineering and industrial biotechnology."
A total of 47 invited lectures in this conference included recent and important topics, for instance, "Synthetic biology for synthetic fuels" by Dr. Jay Keasling from the Joint BioEnergy Institute (USA), "Microbial oil production from renewable feedstocks" by Dr. Greg Stephanopoulos from MIT (USA), "Yeast as a platform cell factory for production of fuels and chemicals" by Dr. Jens Nielsen from Chalmers University (Sweden), "Mammalian synthetic biology - from tools to therapies" by Dr. Martin Fussengger from ETH (Switzerland), "Building, modeling, and applications of metabolic and transcriptional regulatory networks at a genome-scale" by Dr. Bernhard Palsson from the University of California - San Diego (USA), "Genome analysis and engineering Eschericha coli for sucrose utilization" by Dr. Lars Nielsen from the University of Queensland (Australia), "Artificial microorganisms by synthetic biology" by Dr. Daniel Gibson from JCVI (USA), and "Metabolomics and its applications" by Dr. Masaru Tomita from Keio University (Japan). From Korea, Dr. Jin Hwan Park from the research group of Dr. Sang Yup Lee at KAIST presented "Systems metabolic engineering of Escherichia coli for amino acid production," and Dr. Ji Hyun Kim from KRIBB presented "Genome sequencing and omics systems analysis of the protein cell factory of Escherichia coli".
Global companies involved in biorefinery presented their recent research outcomes with emphasis on commercialized technologies. They included "Metabolic and process engineering for commercial outcomes" by Dr. William Provine from DuPont (USA), "Direct production of 1,4-butanediol from renewable feedstocks" by Dr. Mark Burk from Genomatica (USA), "Development of an economically sustainable bioprocess for the production of bio 1,2-propanediol" by Dr. Francis Voelker from Metabolic Explorer (France), "Biotechnology to the bottom-line: low pH lactic acid production at industrial scale" by Dr. Pirkko Suominen from Cargill (USA), "Bioisoprene™: traditional monomer, traditional chemistry, sustainable source" by Dr. Gregg Whited from Danisco (USA) and "Efficient production of pharmaceuticals by engineered fungi" by Dr. Roel Bovenberg from DSM (Netherlands).
This biennial conference also presented the International Metabolic Engineering Award (expanded version of the previous Merck Metabolic Engineering Award) to the best metabolic engineer in the world. The 2010 International Metabolic Engineering Award went to Dr. E. Terry Papoutsakis from the University of Delaware (USA) who has contributed to the production of biobutanol through the metabolic engineering of Clostridia in the last three decades, and he gave an award lecture. Dr. Sang Yup Lee, the current chair of the upcoming conference, was the previous recipient of this award at the last metabolic engineering conference in 2008.
In addition to the invited lectures, a total of 156 carefully selected poster papers were chosen for presentation, and awards were presented to the best posters after rigorous review by the committee members. Such awards included "The 2010 Metabolic Engineering Best Poster Award" and the "2010 Young Metabolic Engineer Award" from the Metabolic Engineering conference, and prestigious international journal awards, including "Wiley Biotechnology Journal Best Poster Award", "Wiley Biotechnology and Bioengineering Best Poster Award" and "Elsevier Metabolic Engineering Best Paper Award." Dr. Catherine Goodman, a senior editor of Nature Chemical Biology, also presented the "Nature Chemical Biology Best Poster Award on Metabolic Engineering."
Regarding this conference, Dr. Sang Yup Lee, the conference chair, said, "This conference is the best international conference in the field of metabolic engineering, which is held every two years, and Korea is the first Asian country to host it. All the experts and students spend time together from early breakfast to late poster sessions, which is a distinct feature of this conference. Although the number of delegates had typically been limited to 200, around 300 delegates were selected this year to accept more attendees from many people who have been interested in metabolic engineering. Also, it is very fitting that "green growth" is the main topic of this conference because Korea is playing a key role in this field. I"m grateful to the Lotte Scholarship Foundation, COFCO, GS Caltex, Bioneer, US DOE, US NSF, Daesang, CJ Cheiljedang, Genomatica and DuPont who provided us with generous financial support that allowed the successful organization of this conference."
The conference was organized by the Systems Biology Research Project Team supported by the Ministry of Eduction, Science and Technology (MEST), Microbial Frontier Research Project Group, World Class University Project Group at KAIST, Institute for the BioCentury at KAIST, Korean Society for Biotechnology and Bioengineering, and the Engineering Conference International (ECI) of the United States.
Inquiries: Professor Sang Yup Lee (+82-42-350-3930), industrialbio@gmail.com
2010.06.25 View 17767 -
New drug targeting method for microbial pathogens developed using in silico cell
A ripple effect is expected on the new antibacterial discovery using “in silico” cells
Featured as a journal cover paper of Molecular BioSystems
A research team of Distinguished Professor Sang Yup Lee at KAIST recently constructed an in silico cell of a microbial pathogen that is resistant to antibiotics and developed a new drug targeting method that could effectively disrupt the pathogen"s growth using the in silico cell.
Hyun Uk Kim, a graduate research assistant at the Department of Chemical and Biomolecular Engineering, KAIST, conducted this study as a part of his thesis research, and the study was featured as a journal cover paper in the February issue of Molecular BioSystems this year, published by The Royal Society of Chemistry based in Europe.
It was relatively easy to treat infectious microbes using antibiotics in the past. However, the overdose of antibiotics has caused pathogens to increase their resistance to various antibiotics, and it has become more difficult to cure infectious diseases these days.
A representative microbial pathogen is Acinetobacter baumannaii. Originally isolated from soils and water, this microorganism did not have resistance to antibiotics, and hence it was easy to eradicate them if infected. However, within a decade, this miroorganism has transformed into a dreadful super-bacterium resistant to antibiotics and caused many casualties among the U.S. and French soldiers who were injured from the recent Iraqi war and infected with Acinetobacter baumannaii.
Professor Lee’s group constructed an in silico cell of this A. baumannii by computationally collecting, integrating, and analyzing the biological information of the bacterium, scattered over various databases and literatures, in order to study this organism"s genomic features and system-wide metabolic characteristics. Furthermore, they employed this in silico cell for integrative approaches, including several network analysis and analysis of essential reactions and metabolites, to predict drug targets that effectively disrupt the pathogen"s growth. Final drug targets are the ones that selectively kill pathogens without harming human body.
Here, essential reactions refer to enzymatic reactions required for normal metabolic functioning in organisms, while essential metabolites indicate chemical compounds required in the metabolism for proper functioning, and their removal brings about the effect of simultaneously disrupting their associated enzymes that interact with them.
This study attempted to predict highly reliable drug targets by systematically scanning biological components, including metabolic genes, enzymatic reactions, that constitute an in silico cell in a short period of time.
This research achievement is highly regarded as it, for the first time, systematically scanned essential metabolites for the effective drug targets using the concept of systems biology, and paved the way for a new antibacterial discovery. This study is also expected to contribute to elucidating the infectious mechanism caused by pathogens.
"Although tons of genomic information is poured in at this moment, application research that efficiently converts this preliminary information into actually useful information is still lagged behind. In this regard, this study is meaningful in that medically useful information is generated from the genomic information of Acinetobacter baumannii," says Professor Lee. "In particular, development of this organism"s in silico cell allows generation of new knowledge regarding essential genes and enzymatic reactions under specific conditions," he added.
This study was supported by the Korean Systems Biology Project of the Ministry of Education, Science and Technology, and the patent for the development of in silico cells of microbial pathogens and drug targeting methods has been filed.
[Picture 1 Cells in silico]
[Picture 2 A process of generating drug targets without harming human body while effectively disrupting the growth of a pathogen, after predicting metabolites from in silico cells]
2010.04.05 View 14578