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Discovery of Redox-Switch of KEenzyme Involved in N-Butanol Biosynthesis
Research teams at KAIST and Kyungpook National University (KNU) have succeeded in uncovering the redox-switch of thiolase, a key enzyme for n-butanol production in Clostridium acetobutylicum, one of the best known butanol-producing bacteria.
Biological n-butanol production was first reported by Louis Pasteur in 1861, and the bioprocess was industrialized usingClostridium acetobutylicum. The fermentation process by Clostridium strains has been known to be the most efficient one for n-butanol production. Due to growing world-wide issues such as energy security and climate change, the biological production of n-butanol has been receiving much renewed interest. This is because n-butanol possesses much better fuel characteristics compared to ethanol, such as higher energy content (29.2 MJ/L vs 19.6 MJ/L), less corrosiveness, less hygroscopy, and the ease with which it can be blended with gasoline and diesel.
In the paper published in Nature Communications, a broad-scope, online-only, and open access journal issued by the Nature Publishing Group (NPG), on September 22, 2015, Professor Kyung-Jin Kim at the School of Life Sciences, KNU, and Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering, KAIST, have proved that the redox-switch of thiolase plays a role in a regulation of metabolic flux in C. acetobutylicum by using in silico modeling and simulation tools.
The research team has redesigned thiolase with enhanced activity on the basis of the 3D structure of the wild-type enzyme. To reinforce a metabolic flux toward butanol production, the metabolic network of C. acetobutylicum strain was engineered with the redesigned enzyme. The combination of the discovery of 3D enzyme structure and systems metabolic engineering approaches resulted in increased n-butanol production in C. acetobutylicum, which allows the production of this important industrial chemical to be cost competitive.
Professors Kim and Lee said, "We have reported the 3D structure of C. acetobutylicum thiolase-a key enzyme involved in n-butanol biosynthesis, for the first time. Further study will be done to produce butanol more economically on the basis of the 3D structure of C. acetobutylicum thiolase."
This work was published online in Nature Communications on September 22, 2015.
Reference: Kim et al. "Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum," Nature Communications
This research was supported by the Technology Development Program to Solve Climate Changes from the Ministry of Education, Science and Technology (MEST), Korea, the National Research Foundation of Korea, and the Advanced Biomass Center through the Global Frontier Research Program of the MEST, Korea.
For further information, contact Dr. Sang Yup Lee, Distinguished Professor, KAIST, Daejeon, Korea (leesy@kaist.ac.kr, +82-42-350-3930); and Dr. Kyung-Jin Kim, Professor, KNU, Daegu, Korea (kkim@knu.ac.kr, +82-53-950-6088).
Figure 1: A redox-switch of thiolase involves in butanol biosynthesis in Clostridium acetobutylicum. Thiolase condenses two acetyl-CoA molecules for initiating four carbon flux towards butanol.
Figure 2: Thiolase catalyzes the condensation reaction of acetyl-CoA to acetoacetyl-CoA. Two catalytic cysteine residues at 88th and 378th are oxidized and formed an intermolecular disulfide bond in an oxidized status, which results in inactivation of the enzyme for n-butanol biosynthesis. The intermolecular disulfide bond is broken enabling the n-butanol biosynthesis, when the environment status is reduced.
2015.09.23 View 9849 -
KAIST and Chongqing University of Technology in China Open an International Program
With the help of KAIST, Chongqing University of Technology (CQUT) in China established an electrical engineering and computer science program and admitted their first 66 freshmen this fall semester.
The joint program was created to foster skilled engineers in the fields of electrical engineering and computer science, which are necessary for the development of the Korean and Chinese Industrial Complex located in Chongqing City.
KAIST has provided CQUT with a majority of the program’s curricula currently offered to its students in Daejeon, Korea.
Under the jointly administered program, KAIST takes on education and research while CQUT is responsible for student selection and administration.
KAIST has dispatched eight professors to teach the related fields in English, and 17 CQUT professors will teach the rest of the curricula.
In August 2014, KAIST and CQUT singed a cooperation agreement for education and research exchange and created the CQUT-KAIST Education Cooperation Center, which is headed by Professor Young-Nam Han of the Electrical Engineering Department at KAIST.
The two universities will expand their collaboration to include graduate programs by 2016.
In the picture below, President Steve Kang of KAIST (right) shakes hands with President Shi Xiaohui of Chongqing University of Technology (left).
2015.09.17 View 8672 -
Professor Woontack Woo Demonstrates an Optical Platform Technology for Augmented Reality at Smart Cloud Show
Professor Woontack Woo of the Graduate School of Culture Technology at KAIST participated in the Smart Cloud Show, a technology exhibition, hosted by the university’s Augmented Human Research Center and presented the latest development of his research, an optical platform system for augmented reality.
This event took place on September 16-17, 2015 at Grand Seoul Nine Tree Convention Center in Seoul.
At the event, Professor Woo introduced a smart glass with an embedded augmented reality system, which permits remote collaboration between an avatar and the user’s hand.
The previous remote collaboration was difficult for ordinary users to employ because of its two-dimensional screen and complicated virtual reality system.
However, with the new technology, the camera attached to artificial reality (AR) glasses recognizes the user’s hand and tracks it down to collaborate. The avatar in the virtual space and the user’s hand interact in real space and time.
The key to this technology is the stable, real-time hand-tracking technique that allows the detection of the hand’s locations and the recognition of finger movements even in situations of self-occlusion.
Through this method, a user can touch and manipulate augmented contents as if they were real-life objects, thereby collaborating remotely with another user who is physically distant by linking his or her movements with an avatar.
If this technology is adopted widely, it may bring some economic benefits such as increased productivity due to lower costs for mobility and reduction in social overhead costs from the decrease in the need of traveling long distance.
Professor Woo said, “This technology will provide us with a greater opportunity for collaboration, not necessarily restricted to physical travelling, which can be widely used in the fields of medicine, education, entertainment, and tourism.”
Professor Woo plans to present his research results on hand-movement tracking and detection at the 12th International Conference on Ubiquitous Robots and Ambient Intelligence (URAI 2015), to be held on October 28-30, 2015, at Kintex in Goyang, Korea.
He will also present a research paper on remote collaboration at the ICAT-EGVE 2015 conference, the merger of the 25th International Conference on Artificial Reality and Telexistence (ICAT 2015) and the 20th Eurographics Symposium on Virtual Environments (EGVE 2015), which will take place on October 28-30, 2015 at the Kyoto International Community House, Kyoto, Japan.
2015.09.16 View 7662 -
KAIST Participates in the World Economic Forum's Annual Meeting of the New Champions 2015 in China
KAIST’s president and its professors actively engage in discussions of major issues on higher education, technology innovation, and industry-university collaboration with global leaders from across all sectors.
President Steve Kang of KAIST participated in the Annual Meeting of the New Champions 2015 (a.k.a., Summer Davos Forum) hosted by the World Economic Forum (WEF). With the theme of “Charting a New Course for Growth,” the Summer Davos Forum took place on September 9-11, 2015 in, Dalian, China.
Currently, KAIST is a member of the Global University Leaders Forum (GULF) of WEF, a gathering of the presidents of the top 25 universities in the world, including Harvard University, Massachusetts Institute of Technology, University of Tokyo, University of Oxford, Peking University, and National University of Singapore. GULF allows university leaders an opportunity to have high-level dialogues on higher education and research and explore prospects for cooperative ventures.
President Kang led the discussion of the GULF session at the Summer Davos Forum, which was held on September 10, 2015, with 25 university leaders as well as two business leaders from Chinese companies: Huawei Technologies Co. Ltd., and Sanofi China. The participants shared candid perspectives on industry-university collaboration, particularly the need for such partnerships in Asia.
In addition, KAIST hosted the fourth IdeasLab session, entitled “Bio versus Nano Materials, on September 9, 2015. At the session, four KAIST professors held an in-depth debate and discussion with the audience on whether the next industrial revolution would be driven by advances in biomaterials or nanomaterials.
The topics under discussion were:
- New materials that mimic biology by Professor Hea Shin Lee
- Bio-based materials that replace petroleum-based materials by Professor Sang Yup Lee
- New materials designed at sub-nano scale by Professor Hee Tae Jung
- A hydrogen economy with nanomaterials by Professor Eun Ae Cho
Since its establishment in 2007, the Summer Davos Forum has become the biggest business and political gathering in Asia, held annually either in Dalian or Tianjin, China. The Forum has attracted more than 1,500 participants primarily from emerging nations such as China, India, Russia, Mexico, and Brazil, and has offered an open platform to address issues important to the region and the global community.
2015.09.14 View 7506 -
KAIST to Hold a Colloquium on the Internet of Things and Open Stack
With the support of the Ministry of Science, ICT, and Future Planning of Korea, KAIST hosted a colloquium on the Internet of Things (IoT) and Open Stack at the KAIST Research Center for Global Cooperation located in Pangyo. The upcoming event was organized to provide an overview of the technological trends to IT companies and foster their success in the global market.
The colloquium invited numerous field-experts as speakers and discussed the influence of IoT and OpenStack on the small- and medium-sized companies in Korea.
Professor Gwan-Hoo Lee from the American University joined as a speaker and shared his insights of how IoT would change the global business environment. He introduced various business models developed by Microsoft, Intel, Apple, Google, and Cisco and discussed how Korean companies could utilize the existing tools and strategy to succeed in the global market. His talk focused specifically on the importance of overseas partnerships and technology stack analysis.
President Hyun-Jung Jang from the Korean OpenStack Community also gave a speech on global partnership through OpenStack. He discussed the future trends of OpenStack and why companies should invest in the field.
KAIST used the event as an opportunity to provide information to domestic companies that are interested in building partnerships with overseas companies and in developing new IoT and OpenStack technology.
More information about the event can be provided online at www.ictc.or.kr.
2015.09.11 View 4753 -
Professor Jeong Ho Lee Receives the 2015 Pediatric Epilepsies Research Award
The award identifies leading scientists worldwide and funds their cutting-edge research in epilepsy.
The Citizen United for Research in Epilepsy (CURE) announced on September 7, 2015, that Jeong Ho Lee, a professor of the Graduate School of Medical Science and Engineering at KAIST, will be awarded the 2015 Pediatric Epilepsies Research Award.
The Pediatric Epilepsies Research Award is given annually to a researcher who has conducted novel, innovative research projects that address severe, intractable pediatric epilepsies as well as collaborative, interdisciplinary projects that explore new approaches to find a treatment for pediatric epilepsies.
Lee was recognized for his leading study in the field of intractable epilepsy. He is the first Korean who has ever received this award, securing a research grant of USD 250,000 for two years.
Lee has conducted research on brain somatic mutations as the novel cause of childhood intractable epilepsy. Pediatric epilepsies account for approximately 70% of all cases of epilepsy.
Established in 1998, CURE is a non-profit American organization based in Chicago, Illinois, which is committed to funding research and various initiatives that will lead to breakthroughs to cure epilepsy.
Since its inception, CURE has been at the forefront of epilepsy research, raising more than USD 32 million to support researchers and scientists worldwide. It has also awarded more than 180 cutting-edge projects in 13 countries.
2015.09.09 View 10294 -
KAIST's Student Job Fair 2015
KAIST’s Undergraduate Student Council and Graduate Student Council jointly hosted the 2015 KAIST Job Fair on September 2-3, 2015 at the Sports Complex on campus.
The Job Fair took place for the sixth time this year. Forty-three companies, including some of the largest ones in Korea such as Samsung, Hyundai, LG, SK Construction, Hankook Tires, as well as those owned by KAIST graduates, have participated.
The Job Fair specialized in three fields: information technology (IT) and electronic and mechanical engineering. The event included one-to-one employment counseling between human resources managers and students, mock interviews, employment orientations, job consulting, interview makeup lessons, resume writing and photo-shooting, etc.
The international students who attended the event received information packages on employment from the participating companies.
This event was open not only to KAIST students but also to students from other universities in the local community, offering more job opportunities to a wider range of people.
Last year alone, a total of 1,200 people including KAIST students and graduates joined the KAIST’s Job Fair.
2015.09.04 View 5589 -
Professor Poong-Hyun Seong Appointed as the New President of the Korean Nuclear Society
Professor Poong-Hyun Seong of the Department of Nuclear and Quantum Engineering has been appointed as the 28th President of the Korean Nuclear Society (KNS). His presidency began on September 1, 2015 and will last one year.
Professor Seong graduated from the Department of Nuclear Engineering at Seoul National University, Korea, and received his master’s and doctoral degree at the Massachusetts Institute of Technology (MIT) in the United States.
He has filled various positions in the field of nuclear engineering, including a commissioner of the Nuclear Safety and Security Commission of Korea, manager of the Korean branch of the American Nuclear Society (ANS), and head of the Human Factors and Instrumentation and Controls Division of ANS.
At KNS, he served as the vice president as well as the Editor-in-Chief of its academic journal Nuclear Engineering and Technology.
The Korean Nuclear Society was established in 1969 to promote academic and technical research in nuclear engineering and develop safe and sustainable nuclear power. It is composed of more than 4,200 active members from ten research fields and has published the journal Nuclear Engineering and Technology since 2007.
2015.09.01 View 5360 -
KAIST's Research Team Receives the Best Paper Award from the IEEE Transaction on Power Electronics
A research team led by Professor Chun T. Rim of the Department of Nuclear and Quantum Engineering at the Korea Advanced Institute of Science and Technology (KAIST) has received the First Prize Papers Award from the IEEE (Institute of Electrical and Electronics Engineers) Transactions on Power Electronics (TPEL), a peer-reviewed journal that covers fundamental technologies used in the control and conversion of electric power.
A total of three research papers received this award in 2015.
Each year, TPEL’s editors select three best papers among those published in the journal during the preceding calendar year. In 2014, the TPEL published 579 papers. Professor Rim’s paper was picked out as one of the three papers published last year for the First Prize Papers Award.
Entitled “Generalized Active EMF (electromagnetic field) Cancel Methods for Wireless Electric Vehicles (http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6684288&tag=1),” the paper proposed, for the first time in the world, three generalized design methods for cancelling the total EMF generated from wireless electric vehicles. This technology, researchers said, can be applied to any wireless power transfer systems.
The award ceremony will be held at the upcoming conference of the 2015 IEEE Energy Conversion Congress and Expo in September in Montreal, Canada.
2015.08.27 View 8818 -
Nature Biotechnology Nominates Sang Yup Lee of KAIST for Top 20 Translational Researchers of 2014
Nature Biotechnology, recognized as the most prestigious journal in the field of biotechnology, has released today its list of the Top 20 Translational Researchers of 2014. Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at KAIST (Korea Advanced Institute of Science and Technology) ranked seventh in the list. He is the only Asian researcher listed.
The journal, in partnership with IP Checkups, a patent analytics firm, presents an annual ranking of researchers based on their paper and patent output. The list includes, among others, each researcher’s most-cited patent in the past five years and their H index, a measurement to evaluate the impact of a researcher’s published work utilizing citation analysis. (More details can be found at http://www.nature.com/bioent/2015/150801/full/bioe.2015.9.html.)
American institutions made up the majority of the list, with 18 universities and research institutes, and the remainder was filled by KAIST in Korea and the Commonwealth Scientific and Industrial Research Organization (CSIRO) in Australia.
Globally known as a leading researcher in systems metabolic engineering, Professor Lee has published more than 500 journal papers and 580 patents. He has received many awards, including the Citation Classic Award, Elmer Gaden Award, Merck Metabolic Engineering Award, ACS Marvin Johnson Award, SIMB Charles Thom Award, POSCO TJ Park Prize, Amgen Biochemical Engineering Award, and the Ho Am Prize in Engineering.
2015.08.27 View 8872 -
'Engineered Bacterium Produces 1,3-Diaminopropane'
A research team led by Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at KAIST reported, for the first time, the production of 1,3-diaminopropane via fermentation of an engineered bacterium.
1,3-Diaminopropane is a three carbon diamine, which has a wide range of industrial applications including epoxy resin and cross-linking agents, as well as precursors for pharmaceuticals, agrochemicals, and organic chemicals. It can also be polymerized with dicarboxylic acids to make polyamides (nylons) for use as engineering plastics, medical materials, and adhesives.
Traditionally, 1,3-diaminopropane is derived from petroleum-based processes. In effort to address critical problems such as the depletion of petroleum and environmental issues inherent to the petroleum-based processes, the research team has developed an Escherichia coli (E. coli) strain capable of producing 1,3-diaminopropane. Using this technology, 1,3-diaminopropane can now be produced from renewable biomass instead of petroleum.
E. coli as found in nature is unable to produce 1,3-diaminopropane. Metabolic engineering, a technology to transform microorganisms into highly efficient microbial cell factories capable of producing chemical compounds of interest, was utilized to engineer the E. coli strain. First, naturally existing metabolic pathways for the biosynthesis of 1,3-diaminopropane were introduced into a virtual cell in silico to determine the most efficient metabolic pathway for the 1,3-diaminopropane production. The metabolic pathway selected was then introduced into an E. coli strain and successfully produced 1,3-diaminopropane for the first time in the world.
The research team applied metabolic engineering additionally, and the production titer of 1,3-diaminopropane increased about 21 fold. The Fed-batch fermentation of the engineered E. coli strain produced 13 grams per liter of 1,3-diaminoproapne. With this technology, 1,3-diaminopropane can be produced using renewable biomass, and it will be the starting point for replacing the current petroleum-based processes with bio-based processes.
Professor Lee said, “Our study suggested a possibility to produce 1,3-diaminopropane based on biorefinery. Further study will be done to increase the titer and productivity of 1,3-diaminopropane.”
This work was published online in Scientific Reports on August 11, 2015.
Reference: Chae, T.U. et al. "Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine," Scientific Reports:
http://www.nature.com/articles/srep13040
This research was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea.
Figure 1: Metabolic engineering strategies for 1,3-diaminopropane production using C4 pathway
Figure 2: Fed-batch fermentation profiles of two final engineered E. coli strains
2015.08.12 View 10131 -
Professor Sang-Min Bae Receives the 2015 Red Dot Design Award
Professor Sang-Min Bae and his research team from the Industrial Design Department of KAIST have received three awards from the 2015 Red Dot Design Award: the Best of the Best Award and two Design Concept Awards.
Being one of the most prestigious international design awards, the Red Dot Design Award serves to identify good design concept and innovation that will be the precursors of tomorrow’s great product. Its annual award ceremony will take place on September 25, 2015, at the Red Dot Design Museum in Singapore.
This year, the Award received 4,680 entries from 61 countries, and only the top 5.7% of them was able to garner the awards. In addition to two Red Dot Design Concept Awards, Professor Bae’s team won the Best of the Best Award, coming through a four hundred to one competition.
Awarded the Best of the Best Award, Boxchool is a modular classroom built on shipping containers whose aim is to give underprivileged children equal opportunities for learning. Jointly designed with an IT corporation in Korea, SK Telecom, the container is also a smart classroom.
Boxchool received the Best of the Best Award in recognition of its contribution to giving an equal learning opportunity to needy children, as well as its environmental characteristics.
The research team strengthened the mobility of the container and creatively addressed problems associated with using containers as classrooms such as insulation and inadequate space in environments which hamper teaching. The modular classroom can function in any setting since it can generate electricity from installed solar panels. The rainwater utilization system allows autonomous operation of the classroom.
The team earned the Red Dot Design Concept Award for a self-generating interactive tent, which was jointly designed with Kolon Sport, a Korean outdoor products company, as an industry-university cooperation project. The interactive tent differs from conventional tents by adding features that allow users to engage with the environment. For example, the installed organic solar cells allowed users to have prolonged outdoor activities by supplying electricity generated therefrom. Users can also enjoy greater ventilation. This permits the tent to be utilized as a temporary residence in the third world.
Another recipient of the Red Dot Design Concept Award, Snow Energy is a portable self-generating lamp and charger, which contains a thermo-element, generating electricity from temperature difference. Electricity is generated by pouring hot water inside a tank and cold water into a neighboring space. Snow Energy, which is sustainable and eco-friendly, will be especially helpful during outdoor activities when there is no electricity available.
Professor Bae's research team, ID+IM, has endeavored to address inequality and philanthropy through two projects, the Nanum (a Korean word to mean “sharing”) and the Seed Projects. Since 2005, they have received internationally recognized awards more than 40 times.
Picture 1: Recipient of the Best of the Best Award of the 2015 Red Dot Design Award, Boxchool is a modular classroom built on shipping containers
Picture 2: Recipient of the 2015 Red Dot Design Concept Award, the self-generating interactive tent
Picture 3: Recipient of the 2015 Red Dot Design Concept Award, Snow Energy is a portable self-generating lamp and charger which generates electricity from the temperature difference
2015.08.05 View 10452