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Ultra-High Strength Metamaterial Developed Using Graphene
New metamaterial has been developed, exhibiting hundreds of times greater strength than pure metals.
Professor Seung Min, Han and Yoo Sung, Jeong (Graduate School of Energy, Environment, Water, and Sustainability (EEWS)) and Professor Seok Woo, Jeon (Department of Material Science and Engineering) have developed a composite nanomaterial. The nanomaterial consists of graphene inserted in copper and nickel and exhibits strengths 500 times and 180 times, respectively, greater than that of pure metals. The result of the research was published on the July 2nd online edition in Nature Communications journal.
Graphene displays strengths 200 times greater than that of steel, is stretchable, and is flexible. The U.S. Army Armaments Research, Development and Engineering Center developed a graphene-metal nanomaterial but failed to drastically improve the strength of the material.
To maximize the strength increased by the addition of graphene, the KAIST research team created a layered structure of metal and graphene. Using CVD (Chemical Vapor Deposition), the team grew a single layer of graphene on a metal deposited substrate and then deposited another metal layer. They repeated this process to produce a metal-graphene multilayer composite material, utilizing a single layer of graphene. Micro-compression tests within Transmission Electronic Microscope and Molecular Dynamics simulations effectively showed the strength enhancing effect and the dislocation movement in grain boundaries of graphene on an atomic level.
The mechanical characteristics of the graphene layer within the metal-graphene composite material successfully blocked the dislocations and cracks from external damage from traveling inwards. Therefore the composite material displayed strength beyond conventional metal-metal multilayer materials. The copper-graphene multilayer material with an interplanar distance of 70nm exhibited 500 times greater (1.5GPa) strength than pure copper. Nickel-graphene multilayer material with an interplanar distance of 100nm showed 180 times greater (4.0GPa) strength than pure nickel.
It was found that there is a clear relationship between the interplanar distance and the strength of the multilayer material. A smaller interplanar distance made the dislocation movement more difficult and therefore increased the strength of the material. Professor Han, who led the research, commented, “the result is astounding as 0.00004% in weight of graphene increased the strength of the materials by hundreds of times” and “improvements based on this success, especially mass production with roll-to-roll process or metal sintering process in the production of ultra-high strength, lightweight parts for automobile and spacecraft, may become possible.” In addition, Professor Han mentioned that “the new material can be applied to coating materials for nuclear reactor construction or other structural materials requiring high reliability.”
The research project received support from National Research Foundation, Global Frontier Program, KAIST EEWS-KINC Program and KISTI Supercomputer and was a collaborative effort with KISTI (Korea Institute of Science and Technology Information), KBSI (Korea Basic Science Institute), Stanford University, and Columbia University.
A schematic diagram shows the structure of metal-graphene multi-layers. The metal-graphene multi-layered composite materials, containing a single-layered graphene, block the dislocation movement of graphene layers, resulting in a greater strength in the materials.
2013.08.23 View 14275 -
KAIST unveils foldable micro electric car, Armadillo-T
The small and light electric car completely folds in half when parking, making it a perfect fit for public or private transportation in an urban environment.
Looking for a parking space for hours at a busy shopping mall or being stuck on roads jammed with cars releasing large amounts of carbon dioxide are all-too-familiar scenes for city dwellers.
A group of researchers at the Korea Advanced Institute of Science and Technology (KAIST) recently developed a possible solution to such problems: a foldable, compact electric vehicle that can be utilized either as a personal car or part of the public transit system to connect major transportation routes within a city.
In-Soo Suh, associate professor of the Graduate School for Green Transportation at KAIST, and his research team introduced a prototype micro electric car called "Armadillo-T," whose design is based on a native animal of South America, the armadillo, a placental mammal with a leathery armor shell.
The research team imitated the animal"s distinctive protection characteristic of rolling up into a ball when facing with threat from predators. Just as armadillos hide themselves inside the shell, Armadillo-T tucks its rear body away, shrinking its original size of 2.8 meters (110 inches) down to almost half, 1.65 meters (65 inches), when folding.
Armadillo-T is a four-wheel-drive, all-electric car with two seats and four in-wheel motors. Since the motors are installed inside the wheels, and the 13.6 kWh capacity of lithium-ion battery pack is housed on the front side, the battery and motors do not have to change their positions when the car folds. This not only optimizes the energy efficiency but also provides stability and ample room to drivers and passengers.
Once folded, the small and light (weighs 450 kg) electric vehicle takes up only one-third of a 5-meter parking space, the standard parking size in Korea, allowing three of its kind to be parked. With a smartphone-interfaced remote control on the wheels, the vehicle can turn 360 degrees, enhancing drivers" convenience to park the car, even in an odd space in a parking lot, the corner of a building, for example.
Professor In-Soo Suh said, "I expect that people living in cities will eventually shift their preferences from bulky, petro-engine cars to smaller and lighter electric cars. Armadillo-T can be one of the alternatives city drivers can opt for. Particularly, this car is ideal for urban travels, including car-sharing and transit transfer, to offer major transportation links in a city. In addition to the urban application, local near-distance travels such as tourist zones or large buildings can be another example of application."
The concept car has loads of smart features on board, too: the cameras installed inside the car eliminate the need for side mirrors and increase the driver"s ability to see the car"s right and left side, thereby reducing blind spots. With a smartphone, the driver can control Armadillo-T and enable remote folding control. The car has a maximum speed of 60 km/h, and with a ten-minute fast charge, it can run up to 100 km.
Professor Suh explained that the concept of Armadillo-T was originally initiated in 2011 as he focused his research interest on the sub-A segment of personal mobility vehicles (PMVs), which are smaller and lighter than the current compact cars, as a new personalized transport mode.
"In coming years, we will see more mega-size cities established and face more serious environmental problems. Throughout the world, the aging population is rapidly growing as well. To cope with climate, energy, and limited petroleum resources, we really need to think outside the box, once again, to find more convenient and eco-friendly transportation, just as the Ford Model T did in the early 1920s. A further level of R&D, technical standards, and regulatory reviews are required to have these types of micro vehicles or PMVs on the market through test-bed evaluations, but we believe that Armadillo-T is an icon toward the future transport system with technology innovation."
The research project has been supported by the Korean government, the Ministry of Land, Infrastructure and Transport and the Korea Agency for Infrastructure Technology Advancement, since December 2012.Youtube Link: http://www.youtube.com/watch?v=8DoZH7Y-sR0
2013.08.21 View 13973 -
KAIST's HUBO Ready for DARPA's Robotics Challenge Trials
When walking on muddy or bumpy roads, the two arms of DRC-HUBO become extra legs, enabling stable and agile movements.
The Humanoid Robot Research Center (HUBO Lab, http://hubolab.kaist.ac.kr) at the Korea Advanced Institute of Science and Technology (KAIST) and Rainbow Co., a spin-off venture company of the university, unveiled a new model of HUBO that will be entered in an international robotics competition scheduled later this year.
The competition is hosted and sponsored by the US Defense Advanced Research Projects Agency (DARPA), which is called the DARPA Robotics Challenge (DRC). Kicked off in October 2012, the DRC’s goal is to spur the development of advanced robots that can assist humans in mitigating and recovering from future natural and man-made disasters.
KAIST’s humanoid robot, HUBO, was originally created by Jun-Ho Oh, a distinguished professor of the Department of Mechanical Engineering, in 2004. Since then, the robot has gone through technological advancements, with the latest version of HUBO II released in 2012. So far, 12 HUBOs have been exported for further studies in robotics to universities, research institutes, and private companies in the US, China, and Singapore.
In tandem with Rainbow Co. (www.rainbow-robot.com), Professor Oh and his research team recently developed DRC-HUBO, which will compete as Team DRC-HUBO led by Drexel University at the DRC trials to be held in December 2013. Team DRC-HUBO is consisted of KAIST and nine US institutions.
DRC-HUBO is designed to perform difficult but essential activities required when responding to disaster scenes. The robot will have to fulfill eight tasks assigned by the DRC at the upcoming event such as driving a utility vehicle, walking across rough terrain, climbing a ladder, and using hand tools.
Unlike the previous models of HUBO, DRC-HUBO boasts several distinctive, enhanced features. Chief among them is the way the robot interacts with the external environment. Without complex sensors installed throughout the body, DRC-HUBO can control each joint of the arms and legs in compliance with the dynamics dictated by the external environment.
For example, when DRC-HUBO is faced with a rock falling from above while climbing up a ladder, the robot’s arms and legs naturally give in to the force of external changes. Accordingly, as the robot dodges the rock, its body and joints smoothly sway to absorb shock so that the fingers can keep a tight grip on the ladder, and the feet are planted firmly on the rail of the ladder, not losing balance.
In addition, DRC-HUBO can switch from bipedal to quadrupedal walking and vice versa. This provides the robot with greater stability to walk on uneven terrain or to climb up a hill. The robot’s arms and legs are elongated to better meet the challenges demanded by the DRC competition. DRC-HUBO’s two arms swing back and forth to form legs when necessary, thereby walking freely backwards and forwards.
The robot has gotten stronger grip as well. The right hand has four fingers (with one triggering finger that operates independently from the other three fingers), and the left hand has three fingers. All three fingers on both hands are actuated synchronously for gripping. The fingers are sophisticated enough to steer the wheel of a vehicle or grab a ladder to climb up, and strong enough to hold 15 lbs in one hand.
“With a full 34 degrees of freedom (DOF), DRC-HUBO stands 4.7 ft tall and weighs 120 lbs. All in all, the robot has been improved and extensively refurbished from the past models of HUBOs to compete at the DRC Trials. It has better vision and coordination. The legs and arms have become stronger,” said Professor Oh.
“Although the robot is still a prototype, it has important capabilities that can be utilized in advancing humanoid robots in general. One example is the way its arms can be used as extra legs to support the robot’s body, offering more flexibility in providing aid to humans.”
2013.07.25 View 13367 -
A magnetic pen for smartphones adds another level of conveniences
Utilizing existing features on smartphones, the MagPen provides users with a compatible and simple input tool regardless of the type of phones they are using.
A doctoral candidate at the Korea Advanced Institute of Science and Technology (KAIST) developed a magnetically driven pen interface that works both on and around mobile devices. This interface, called the MagPen, can be used for any type of smartphones and tablet computers so long as they have magnetometers embedded in.
Advised by Professor Kwang-yun Wohn of the Graduate School of Culture Technology (GSCT) at KAIST, Sungjae Hwang, a Ph.D. student, created the MagPen in collaboration with Myung-Wook Ahn, a master"s student at the GSCT of KAIST, and Andrea Bianchi, a professor at Sungkyunkwan University.
Almost all mobile devices today provide location-based services, and magnetometers are incorporated in the integrated circuits of smartphones or tablet PCs, functioning as compasses. Taking advantage of built-in magnetometers, Hwang"s team came up with a technology that enabled an input tool for mobile devices such as a capacitive stylus pen to interact more sensitively and effectively with the devices" touch screen. Text and command entered by a stylus pen are expressed better on the screen of mobile devices than those done by human fingers.
The MagPen utilizes magnetometers equipped with smartphones, thus there is no need to build an additional sensing panel for a touchscreen as well as circuits, communication modules, or batteries for the pen. With an application installed on smartphones, it senses and analyzes the magnetic field produced by a permanent magnet embedded in a standard capacitive stylus pen.
Sungjae Hwang said, "Our technology is eco-friendly and very affordable because we are able to improve the expressiveness of the stylus pen without requiring additional hardware beyond those already installed on the current mobile devices. The technology allows smartphone users to enjoy added convenience while no wastes generated."
The MagPen detects the direction at which a stylus pen is pointing; selects colors by dragging the pen across smartphone bezel; identifies pens with different magnetic properties; recognizes pen-spinning gestures; and estimates the finger pressure applied to the pen.
Notably, with its spinning motion, the MagPen expands the scope of input gestures recognized by a stylus pen beyond its existing vocabularies of gestures and techniques such as titling, hovering, and varying pressures. The tip of the pen switches from a pointer to an eraser and vice versa when spinning. Or, it can choose the thickness of the lines drawn on a screen by spinning.
"It"s quite remarkable to see that the MagPen can understand spinning motion. It"s like the pen changes its living environment from two dimensions to three dimensions. This is the most creative characteristic of our technology," added Sungjae Hwang.
Hwang"s initial research result was first presented at the International Conference on Intelligent User Interfaces organized by the Association for Computing Machinery and held on March 19-22 in Santa Monica, the US.
In the next month of August, the research team will present a paper on the MagPen technology, entitled "MagPen: Magnetically Driven Pen Interaction On and Around Conventional Smartphones" and receive an Honorable Mention Award at the 15th International Conference on Human-Computer Interaction with Mobile Devices and Services (MobileHCI 2013) to be held in Germany.
In addition to the MagPen, Hwang and his team are conducting other projects to develop different types of magnetic gadgets (collectively called "MagGetz") that include the Magnetic Marionette, a magnetic cover for a smartphone, which offers augmented interactions with the phone, as well as magnetic widgets such as buttons and toggle interface.
Hwang has filed ten patents for the MagGetz technology.
Youtube Links: http://www.youtube.com/watch?v=NkPo2las7wc, http://www.youtube.com/watch?v=J9GtgyzoZmM
2013.07.25 View 9933 -
Thinking Out of the Box: KAIST Silicon Valley Innovation Platform
KAIST established a liaison office in San Jose, California, to support the entrepreneurship of KAIST graduates, students, and faculty who aspire to transform their innovative ideas into business.
The office, KAIST Silicon Valley Innovation Platform (SVIP), is located within the Korea Trade-Investment Promotion Agency (KOTRA) IT Center on North First Street in San Jose.
SVIP collects information and analyzes trends on emerging technologies; provides various educational programs on entrepreneurship and technology translation; offers opportunities to prospective entrepreneurs to engage with industry and research and government organizations; and assists Korean startups in accessing the US and North American market.
President Steve Kang attended the opening ceremony of the office on June 14th and encouraged KAIST alumni living in the US to share their ideas and technology innovations and transform them into business opportunities.
For more information, please contact Professor Soung-Hie Kim (seekim@business.kaist.ac.kr) from the Graduate School of Information and Media Management, KAIST.
2013.07.04 View 8078 -
KAIST Placed 3rd in the World's Best 100 Emerging Universities
The Times Higher Education (THE) published its world university rankings on June 20, 2013. It is a list of the best 100 universities whose histories are 50 years old or younger. KAIST was ranked 3rd in the list, two places up from the 5th last year. Forbes and Reuters carried a story on the top ten emerging universities out of the listed 100 institutions, highlighting strong showings in the Asian region. For the articles, please see the attached file (Forbes) or click the link (Reuters) below:
http://www.reuters.com/article/2013/06/19/education-university-rankings-idUSL2N0EU1HZ20130619
2013.06.22 View 7049 -
Technology for Non-Breaking Smartphone Display Developed
High-strength plastic display has been developed by applying a glass-fiber fabric.
“Will bring about innovation to the field by replacing glass substrates”
It is now possible to manufacture non-breaking smartphone display. Heavy glass substrates of large-screen televisions will be replaced with light plastic films.
Professor Choon Sup Yoon from KAIST’s Department of Physics and KAIST Institute for Information Technology Convergence has developed the technology for high-strength plastic substrates to replace glass displays.
The plastic substrate created by Professor Yoon and his research team have greatly enhanced needed properties of heat resistance, transparency, flexibility, inner chemical capability, and tensile strength. Although the material retains flexibility as a native advantage of plastic film, its tensile strength is three times greater than that of normal glass, which is a degree similar to tempered glass. In addition, Professor Yoon’s substrate is as colorless and transparent as glass and resists heat up to 450℃, while its thermal expansivity is only 10% to 20% of existing plastics.
Glass substrates are currently used in practically every display such as mobile phone screens, televisions, and computer monitors for having smooth surface and satisfying basic conditions for display substrates. However, as glass substrates are heavy and easily broken, researchers studied colorless and transparent plastic polyimide films to replace glass substrates for their excellent thermal and chemical stability.
Nonetheless, colorless and transparent polyimide films do not have sufficient heat resistance and mechanical solidity. To resolve this problem, polyimide films are impregnated with glass-fiber fabrics, but it was far from commercialization as the impregnation exacerbates the roughness of surface and light transmittance. The roughness of the surface increases as the solvent evaporates in the impregnation process, resulting in surface roughness of around 0.4μm. The downturn in light transmittance is due to light scattering effect by the discording refractive index of polyimide film and glass-fiber fabric.
Professor Yoon’s research team resolved these issues by tuning the refractive indices of transparent polyimide film and glass-fiber fabric up to four decimal places, and by developing the technology of flattening the film’s surface roughness to a few nanometers. As a result, the research team achieved heat expansivity of 11ppm/℃, surface roughness of 0.9nm, tensile strength of 250MPa, bending curvature radius of 2mm, and light transmittance at 90% with a 110μm-thick glass-fiber fabric impregnated transparent polyimide film substrate.
“The developed substrate can not only replace the traditional glass substrate but also be applied as flexible display substrate,” said Professor Yoon in prospect, “it will bring about technological innovation in display industry as it can fundamentally resolve the issue of shattering mobile phone displays, reduce the weight and thickness of large-area televisions, and apply Roll to Roll process in display manufacture.”
Supported by the Ministry of Knowledge Economy for five years, the technology has applied for 3 patents and is in discussion for technology transfer with related business.
Figure 1. The according (left) and discording (right) refractive indices of glass-fiber fabric and polyimide film. The characters on the left are sharp and clear, but the characters on the right appear foggy.
Figure 2. Picture of the developed glass-fiber fabric
2013.06.09 View 8508 -
Distinguished Professor Sang-Yup Lee received 2013 Amgen Biochemical Engineering Award
- Previous award winners are world-renowned scholars of biochemical engineering including James Bailey, Michael Shuler and Daniel Wang
KAIST Chemical and Biomolecular Engineering Department’s Professor Sang-Yup Lee has been selected to receive the 2013 Amgen Biochemical Engineering Award. The award ceremony will take place this June at the International Biochemical and Molecular Engineering conference in Beijing, China.
The Amgen Biochemical Engineering Award was established by Amgen, a world renowned American pharmaceutical company, in 1993. Amgen awards leading biochemical engineers every two years. The first Amgen award recipient was James Bailey of the California Institute of Technology (Caltech) in 1993. Since then leading engineers that are sometimes called “founding fathers of biochemical engineering” have received the award including MIT Professor Daniel Wang and Michael Shuler of Cornell University.
The first nine award winners were Americans and in 2011 Jens Nielson of Chalmers University of Technology, Sweden, received the Amgen award as a non-American. Professor Sang-Yup Lee is the first Asian to receive the award.
The Amgen award panel said, “Professor Lee made an incredible contribution to the fields of synthetic biology and industrial bioengineering by finding chemical material, fuel, protein and drug production and system bioengineering through metabolic engineering of microorganisms.”
Professor Lee is an expert in metabolic engineering of microorganisms and contributed to the development of system metabolic engineering and system bioengineering. Furthermore, he developed various medical and chemical products and processes which were then applied to synthesise strains of succinate, plastics, butanol and nylon.
Professor Lee is a fellow of the Korean Academy of Science and Technology and National Academy Engineering of Korea; an international member of National Academy of Engineering (US); a former fellow of the American Association for the Advancement of Science; a member of the American Institute of Chemical Engineers, the American Industrial Microbiology Society and American Academy of Microbiology. He is currently Head of Global Agenda Council on Biotechnology and is world renowned for his work in biotechnology field.
2013.04.30 View 8190 -
New Structural Insight into Neurodegenerative Disease
A research team from the Korea Advanced Institute of Science and Technology (KAIST) released their results on the structure and molecular details of the neurodegenerative disease-associated protein Ataxin-1. Mutations in Ataxin-1 cause the neurological disease, Spinocerebella Ataxia Type 1 (SCA1), which is characterized by a loss of muscular coordination and balance (ataxia), as is seen in Parkinson’s, Alzheimer’s, and Huntington’s diseases.
SCA1-causing mutations in the ATAXIN1 gene alter the length of a glutamine stretch in the Ataxin-1 protein. The research team provides the first structural insight into the complex formation of ATAXIN-1 with its binding partner, Capicua (CIC). The team, led by Professor Ji-Joon Song from the Department of Biological Sciences at KAIST, solved the structure of Ataxin-1 and CIC complex in atomic level revealing molecular details of the interaction between Ataxin-1 and CIC.
Professor Song explained his recent research work,
“We are able to see the intricate process of complex formation and reconfiguration of the two proteins when they interact with each other. Our work, we expect, will provide a new therapeutic target to modulate SCA1 neurodegenerative disease.”
Understanding structural and molecular details of proteins at the atomic level will help researchers to track the molecular pathogenesis of the disease and, ultimately, design targeted therapies or treatments for patients, rather than just relieving the symptoms of diseases.
Professor Song’s research paper, entitled “Structural Basis of Protein Complex Formation and Reconfiguration by Polyglutamine Disease Protein ATAXIN-1 and Capicua,” will be published in the March 15th issue of Genes & Development (www.genesdev.org).
Complex Formation and Reconfiguration of ATAXIN-1 and Capicua
The complex formation between a polyglutamine disease protein, ATXIN-1 and the transcriptional repressor Capicua (CIC) plays a critical role in SCA 1 pathogenesis. The image shows that the homodimerization of ATXIN-1 (yellow and red) is disrupted upon binding of CIC (blue). Furthermore, the binding of CIC to the ATXIN-1 induces a new form of ATXIN-1 dimerization mediated by CICs (ATXIN-1 AXH domains are shown in yellow and red, and CIC peptides shown in blue and white).
2013.04.02 View 8557 -
The new era of personalized cancer diagnosis and treatment
Professor Tae-Young Yoon
- Succeeded in observing carcinogenic protein at the molecular level
- “Paved the way to customized cancer treatment through accurate analysis of carcinogenic protein”
The joint KAIST research team of Professor Tae Young Yoon of the Department of Physics and Professor Won Do Huh of the Department of Biological Sciences have developed the technology to monitor characteristics of carcinogenic protein in cancer tissue – for the first time in the world.
The technology makes it possible to analyse the mechanism of cancer development through a small amount of carcinogenic protein from a cancer patient. Therefore, a personalised approach to diagnosis and treatment using the knowledge of the specific mechanism of cancer development in the patient may be possible in the future.
Until recently, modern medicine could only speculate on the cause of cancer through statistics. Although developed countries, such as the United States, are known to use a large sequencing technology that analyses the patient’s DNA, identification of the interactions between proteins responsible for causing cancer remained an unanswered question for a long time in medicine.
Firstly, Professor Yoon’s research team has developed a fluorescent microscope that can observe even a single molecule. Then, the “Immunoprecipitation method”, a technology to extract a specific protein exploiting the high affinity between antigens and antibodies was developed. Using this technology and the microscope, “Real-Time Single Molecule co-Immunoprecipitation Method” was created. In this way, the team succeeded in observing the interactions between carcinogenic and other proteins at a molecular level, in real time.
To validate the developed technology, the team investigated Ras, a carcinogenic protein; its mutation statistically is known to cause around 30% of cancers.
The experimental results confirmed that 30-50% of Ras protein was expressed in mouse tumour and human cancer cells. In normal cells, less than 5% of Ras protein was expressed. Thus, the experiment showed that unusual increase in activation of Ras protein induces cancer.
The increase in the ratio of active Ras protein can be inferred from existing research data but the measurement of specific numerical data has never been done before.
The team suggested a new molecular level diagnosis technique of identifying the progress of cancer in patients through measuring the percentage of activated carcinogenic protein in cancer tissue.
Professor Yoon Tae-young said, “This newly developed technology does not require a separate procedure of protein expression or refining, hence the existing proteins in real biological tissues or cancer cells can be observed directly.” He also said, “Since carcinogenic protein can be analyzed accurately, it has opened up the path to customized cancer treatment in the future.”
“Since the observation is possible on a molecular level, the technology confers the advantage that researchers can carry out various examinations on a small sample of the cancer patient.” He added, “The clinical trial will start in December 2012 and in a few years customized cancer diagnosis and treatment will be possible.”
Meanwhile, the research has been published in Nature Communications (February 19). Many researchers from various fields have participated, regardless of the differences in their speciality, and successfully produced interdisciplinary research. Professor Tae Young Yoon of the Department of Physics and Professors Dae Sik Lim and Won Do Huh of Biological Sciences at KAIST, and Professor Chang Bong Hyun of Computational Science of KIAS contributed to developing the technique.
Figure 1: Schematic diagram of observed interactions at the molecular level in real time using fluorescent microscope. The carcinogenic protein from a mouse tumour is fixed on the microchip, and its molecular characteristics are observed live.
Figure 2: Molecular interaction data using a molecular level fluorescent microscope. A signal in the form of spike is shown when two proteins combine. This is monitored live using an Electron Multiplying Charge Coupled Device (EMCCD). It shows signal results in bright dots.
An organism has an immune system as a defence mechanism to foreign intruders. The immune system is activated when unwanted pathogens or foreign protein are in the body. Antibodies form in recognition of the specific antigen to protect itself. Organisms evolved to form antibodies with high specificity to a certain antigen. Antibodies only react to its complementary antigens. The field of molecular biology uses the affinity between antigens and antibodies to extract specific proteins; a technology called immunoprecipitation. Even in a mixture of many proteins, the protein sought can be extracted using antibodies. Thus immunoprecipitation is widely used to detect pathogens or to extract specific proteins.
Technology co-IP is a well-known example that uses immunoprecipitation. The research on interactions between proteins uses co-IP in general. The basis of fixing the antigen on the antibody to extract antigen protein is the same as immunoprecipitation. Then, researchers inject and observe its reaction with the partner protein to observe the interactions and precipitate the antibodies. If the reaction occurs, the partner protein will be found with the antibodies in the precipitations. If not, then the partner protein will not be found. This shows that the two proteins interact.
However, the traditional co-IP can be used to infer the interactions between the two proteins although the information of the dynamics on how the reaction occurs is lost. To overcome these shortcomings, the Real-Time Single Molecule co-IP Method enables observation on individual protein level in real time. Therefore, the significance of the new technique is in making observation of interactions more direct and quantitative.
Additional Figure 1: Comparison between Conventional co-IP and Real-Time Single Molecule co-IP
2013.04.01 View 17218 -
Top Ten Ways Biotechnology Could Improve Our Everyday Life
The Global Agenda Council on Biotechnology, one of the global networks under the World Economic Forum, which is composed of the world’s leading experts in the field of biotechnology, announced on February 25, 2013 that the council has indentified “ten most important biotechnologies” that could help meet rapidly growing demand for energy, food, nutrition, and health. These new technologies, the council said, also have the potential to increase productivity and create new jobs.
“The technologies selected by the members of the Global Agenda Council on Biotechnology represent almost all types of biotechnology.Utilization of waste, personalized medicine,and ocean agricultureare examples of the challenges where biotechnology can offer solutions,”said Sang Yup Lee, Chair of the Global Agenda Council on Biotechnology and Distinguished Professor in the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST). He also added that “the members of the council concluded that regulatory certainty, public perception, and investment are the key enablers for the growth of biotechnology.”
These ideas will be further explored during “Biotechnology Week” at the World Economic Forum’s Blog (http://wef.ch/blog) from Monday, 25 February, 2013. The full list follows below:
Bio-based sustainable production of chemicals, energy, fuels and materials
Through the last century, human activity has depleted approximately half of the world’s reserves of fossil hydrocarbons. These reserves, which took over 600 million years to accumulate, are non-renewable and their extraction, refining and use contribute significantly to human emissions of greenhouse gases and the warming of our planet. In order to sustain human development going forward, a carbon-neutral alternative must be implemented. The key promising technology is biological synthesis; that is, bio-based production of chemicals, fuels and materials from plants that can be re-grown.
Engineering sustainable food production
The continuing increase in our numbers and affluence are posing growing challenges to the ability of humanity to produce adequate food (as well as feed, and now fuel). Although controversial, modern genetic modification of crops has supported growth in agricultural productivity. In 2011, 16.7 million farmers grew biotechnology-developed crops on almost 400 million acres in 29 countries, 19 of which were developing countries. Properly managed, such crops have the potential to lower both pesticide use and tilling which erodes soil.
Sea-water based bio-processes
Over 70% of the earth surface is covered by seawater, and it is the most abundant water source available on the planet. But we are yet to discover the full potential of it. For example with halliophic bacteria capable of growing in the seawater can be engineered to grow faster and produce useful products including chemicals, fuels and polymeric materials. Ocean agriculture is also a promising technology. It is based on the photosynthetic biomass from the oceans, like macroalgae and microalgae.
Non-resource draining zero waste bio-processing
The sustainable goal of zero waste may become a reality with biotechnology. Waste streams can be processed at bio-refineries and turned into valuable chemicals and fuels, thereby closing the loop of production with no net waste. Advances in biotechnology are now allowing lower cost, less draining inputs to be used, including methane, and waste heat. These advances are simplifying waste streams with the potential to reduce toxicity as well as support their use in other processes, moving society progressively closer to the sustainable goal of zero waste.
Using carbon dioxide as a raw material
Biotechnology is poised to contribute solutions to mitigate the growing threat of rising CO2 levels. Recent advances are rapidly increasing our understanding of how living organisms consume and use CO2. By harnessing the power of these natural biological systems, scientists are engineering a new wave of approaches to convert waste CO2 and C1 molecules into energy, fuels, chemicals, and new materials.
Regenerative medicine
Regenerative medicine has become increasingly important due to both increased longevity and treatment of injury. Tissue engineering based on various bio-materials has been developed to speed up the regenerative medicine. Recently, stem cells, especially the induced pluripotent stem cells (iPS), have provided another great opportunity for regenerative medicine. Combination of tissue engineering and stem cell (including iPS) technologies will allow replacements of damaged or old human organs with functional ones in the near future.
Rapid and precise development and manufacturing of medicine and vaccines
A global pandemic remains one of the most real and serious threats to humanity. Biotechnology has the potential to rapidly identify biological threats, develop and manufacture potential cures. Leading edge biotechnology is now offering the potential to rapidly produce therapeutics and vaccines against virtually any target. These technologies, including messenger therapeutics, targeted immunotherapies, conjugated nanoparticles, and structure-based engineering, have already produced candidates with substantial potential to improve human health globally.
Accurate, fast, cheap, and personalized diagnostics and prognostics
Identification of better targets and combining nanotechnology and information technology it will be possible to develop rapid, accurate, personalized and inexpensive diagnostics and prognostics systems.
Bio-tech improvements to soil and water
Arable land and fresh water are two of the most important, yet limited, resources on earth. Abuse and mis-appropriation have threatened these resources, as the demand on them has increased. Advances in biotechnology have already yielded technologies that can restore the vitality and viability of these resources. A new generation of technologies: bio-remediation, bio-regeneration and bio-augmentation are being developed, offering the potential to not only further restore these resources, but also augment their potential.
Advanced healthcare through genome sequencing
It took more than 13 years and $1.5 billion to sequence the first human genome and today we can sequence a complete human genome in a single day for less than $1,000. When we analyze the roughly 3 billion base pairs in such a sequence we find that we differ from each other in several million of these base pairs. In the vast majority of cases these difference do not cause any issues but in rare cases they cause disease, or susceptibility to disease. Medical research and practice will increasingly be driven by our understanding of such genetic variations together with their phenotypic consequences.
2013.03.19 View 11024
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An efficient strategy for developing microbial cell factories by employing synthetic small regulatory RNAs
A new metabolic engineering tool that allows fine control of gene expression level by employing synthetic small regulatory RNAs was developed to efficiently construct microbial cell factories producing desired chemicals and materials
Biotechnologists have been working hard to address the climate change and limited fossil resource issues through the development of sustainable processes for the production of chemicals, fuels and materials from renewable non-food biomass. One promising sustainable technology is the use of microbial cell factories for the efficient production of desired chemicals and materials. When microorganisms are isolated from nature, the performance in producing our desired product is rather poor. That is why metabolic engineering is performed to improve the metabolic and cellular characteristics to achieve enhanced production of desired product at high yield and productivity. Since the performance of microbial cell factory is very important in lowering the overall production cost of the bioprocess, many different strategies and tools have been developed for the metabolic engineering of microorganisms.
One of the big challenges in metabolic engineering is to find the best platform organism and to find those genes to be engineered so as to maximize the production efficiency of the desired chemical. Even Escherichia coli, the most widely utilized simple microorganism, has thousands of genes, the expression of which is highly regulated and interconnected to finely control cellular and metabolic activities. Thus, the complexity of cellular genetic interactions is beyond our intuition and thus it is very difficult to find effective target genes to engineer. Together with gene amplification strategy, gene knockout strategy has been an essential tool in metabolic engineering to redirect the pathway fluxes toward our desired product formation. However, experiment to engineer many genes can be rather difficult due to the time and effort required; for example, gene deletion experiment can take a few weeks depending on the microorganisms. Furthermore, as certain genes are essential or play important roles for the survival of a microorganism, gene knockout experiments cannot be performed. Even worse, there are many different microbial strains one can employ. There are more than 50 different E. coli strains that metabolic engineer can consider to use. Since gene knockout experiment is hard-coded (that is, one should repeat the gene knockout experiments for each strain), the result cannot be easily transferred from one strain to another.
A paper published in Nature Biotechnology online today addresses this issue and suggests a new strategy for identifying gene targets to be knocked out or knocked down through the use of synthetic small RNA. A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), a prestigeous science and engineering university in Korea reported that synthetic small RNA can be employed for finely controlling the expression levels of multiple genes at the translation level. Already well-known for their systems metabolic engineering strategies, Professor Lee’s team added one more strategy to efficiently develop microbial cell factories for the production of chemicals and materials.
Gene expression works like this: the hard-coded blueprint (DNA) is transcribed into messenger RNA (mRNA), and the coding information in mRNA is read to produce protein by ribosomes. Conventional genetic engineering approaches have often targeted modification of the blueprint itself (DNA) to alter organism’s physiological characteristics. Again, engineering the blueprint itself takes much time and effort, and in addition, the results obtained cannot be transferred to another organism without repeating the whole set of experiments. This is why Professor Lee and his colleagues aimed at controlling the gene expression level at the translation stage through the use of synthetic small RNA. They created novel RNAs that can regulate the translation of multiple messenger RNAs (mRNA), and consequently varying the expression levels of multiple genes at the same time. Briefly, synthetic regulatory RNAs interrupt gene expression process from DNA to protein by destroying the messenger RNAs to different yet controllable extents. The advantages of taking this strategy of employing synthetic small regulatory RNAs include simple, easy and high-throughput identification of gene knockout or knockdown targets, fine control of gene expression levels, transferability to many different host strains, and possibility of identifying those gene targets that are essential.
As proof-of-concept demonstration of the usefulness of this strategy, Professor Lee and his colleagues applied it to develop engineered E. coli strains capable of producing an aromatic amino acid tyrosine, which is used for stress symptom relief, food supplements, and precursor for many drugs. They examined a large number of genes in multiple E. coli strains, and developed a highly efficient tyrosine producer. Also, they were able to show that this strategy can be employed to an already metabolically engineered E. coli strain for further improvement by demonstrating the development of highly efficient producer of cadaverine, an important platform chemical for nylon in the chemical industry.
This new strategy, being simple yet very powerful for systems metabolic engineering, is thus expected to facilitate the efficient development of microbial cell factories capable of producing chemicals, fuels and materials from renewable biomass.
Source: Dokyun Na, Seung Min Yoo, Hannah Chung, Hyegwon Park, Jin Hwan Park, and Sang Yup Lee, “Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs”, Nature Biotechnology, doi:10.1038/nbt.2461 (2013)
2013.03.19 View 9352