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Success in Measuring Protein Interaction at the Molecular Level
Professor Tae Young Yoon - Live observation of two protein interaction in molecular level successful- The limit in measurement and time resolution of immunoprecipitation technique improved by a hundred thousand fold KAIST Department of Physics Professor Tae Young Yoon’s research team has successfully observed the interaction of two proteins live on molecular level and the findings were published in the October edition of Nature Protocols. Professor Yoon’s research team developed a fluorescent microscope that can observe a single molecule. The team grafted the immunoprecipitation technique, traditionally used in protein interaction analysis, to the microscope to develop a “live molecular level immunoprecipitation technique”. The team successfully and accurately measured the reaction between two proteins by repeated momentary interactions in the unit of tens of milliseconds. The existing immunoprecipitation technique required at least one day to detect interaction between two proteins. There were limitations in detecting momentary or weak interactions. Also, quantitative analysis of the results was difficult since the image was measured by protein-band strength. The technique could not be used for live observation. The team aimed to drastically improve the existing technique and to develop accurate method of measurement on molecular level. The newly developed technology can enable observation of protein interaction within one hour. Also, the interaction can be measured live, thus the protein interaction phenomenon can be measured in depth. Moreover, every programme used in the experiment was developed and distributed by the research team so source energy is secured and created the foundation for global infra. Professor Tae Young Yoon said, “The newly developed technology does not require additional protein expression or purification. Hence, a very small sample of protein is enough to accurately analyse protein interaction on a kinetic level.” He continued, “Even cancerous protein from the tissue of a cancer patient can be analysed. Thus a platform for customised anti-cancer medicine in the future has been prepared, as well.” Figure 1. Mimetic diagram comparing the existing immunoprecipitation technique and the newly developed live molecular level immunoprecipitation technique
2013.12.11
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Professor Chun-Taek Rim Appointed as Associate Editor for IEEE TPEL
Professor Chun-Taek Rim of the nuclear and quantum engineering at KAIST was appointed as an associate editor of the Institute of Electrical and Electronics Engineers (IEEE) Transactions on Power Electronics (TPEL), an eminent academic journal bio-monthly published in the field of power electronics.The journal has a high impact factor (4.08), a measure reflecting the average number of citations to recent articles published in an academic journal, which ranks as the 6th the most influential journal among the 100 journals published by IEEE.Professor Rim was also appointed to an associate editor for IEEE Journal of Emerging and Selected Topics in Power Electronics in September in recognition of his expertise in wireless power and electric vehicles.
2013.11.15
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A powerful strategy for developing microbial cell factories by employing synthetic small RNAs
The current systems for the production of chemicals, fuels and materials heavily rely on the use of fossil resources. Due to the increasing concerns on climate change and other environmental problems, however, there has been much interest in developing biorefineries for the production of such chemicals, fuels and materials from renewable resources. For the biorefineries to be competitive with the traditional fossil resource-based refineries, development of high performance microorganisms is the most important as it will affect the overall economics of the process most significantly. Metabolic engineering, which can be defined as purposeful modification of cellular metabolic and regulatory networks with an aim to improve the production of a desired product, has been successfully employed to improve the performance of the cell. However, it is not trivial to engineer the cellular metabolism and regulatory circuits in the cell due to their high complexity. In metabolic engineering, it is important to find the genes that need to be amplified and attenuated in order to increase the product formation rate while minimizing the production of undesirable byproducts. Gene knock-out experiments are often performed to delete those metabolic fluxes that will consequently result in the increase of the desired product formation. However, gene knock-out experiments require much effort and time to perform, and are difficult to do for a large number of genes. Furthermore, the gene knock-out experiments performed in one strain cannot be transferred to another organism and thus the whole experimental process has to be repeated. This is a big problem in developing a high performance microbial cell factory because it is required to find the best platform strain among many different strains. Therefore, researchers have been eager to develop a strategy that allows rapid identification of multiple genes to be attenuated in multiple strains at the same time. A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) reported the development of a strategy for efficiently developing microbial cell factories by employing synthetic small RNAs (sRNAs). They first reported the development of such system in Nature Biotechnology last February. This strategy of employing synthetic sRNAs in metabolic engineering has been receiving great interest worldwide as it allows easy, rapid, high-throughput, tunable, and un-doable knock-down of multiple genes in multiple strains at the same time. The research team published a paper online on August 8 as a cover page (September issue) in Nature Protocols, describing the detailed strategy and protocol to employ synthetic sRNAs for metabolic engineering. In this paper, researchers described the detailed step-by-step protocol for synthetic sRNA-based gene expression control, including the sRNA design principles. Tailor-made synthetic sRNAs can be easily manipulated by using conventional gene cloning method. The use of synthetic sRNAs for gene expression regulation provides several advantages such as portability, conditionality, and tunability in high-throughput experiments. Plasmid-based synthetic sRNA expression system does not leave any scar on the chromosome, and can be easily transferred to many other host strains to be examined. Thus, the construction of libraries and examination of different host strains are much easier than the conventional hard-coded gene manipulation systems. Also, the expression of genes can be conditionally repressed by controlling the production of synthetic sRNAs. Synthetic sRNAs possessing different repression efficiencies make it possible to finely tune the gene expression levels as well. Furthermore, synthetic sRNAs allow knock-down of the expression of essential genes, which was not possible by conventional gene knock-out experiments. Synthetic sRNAs can be utilized for diverse experiments where gene expression regulation is needed. One of promising applications is high-throughput screening of the target genes to be manipulated and multiple strains simultaneously to enhance the production of chemicals and materials of interest. Such simultaneous optimization of gene targets and strains has been one of the big challenges in metabolic engineering. Another application is to fine tune the expression of the screened genes for flux optimization, which would enhance chemical production further by balancing the flux between biomass formation and target chemical production. Synthetic sRNAs can also be applied to finely regulating genetic interactions in a circuit or network, which is essential in synthetic biology. Once a sRNA scaffold-harboring plasmid is constructed, tailor-made, synthetic sRNAs can be made within 3-4 days, followed by the desired application experiments. Dr. Eytan Zlotorynski, an editor at Nature Protocols, said "This paper describes the detailed protocol for the design and applications of synthetic sRNA. The method, which has many advantages, is likely to become common practice, and prove useful for metabolic engineering and synthetic biology studies." This paper published in Nature Protocols will be useful for all researchers in academia and industry who are interested in the use of synthetic sRNAs for fundamental and applied biological and biotechnological studies. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) and the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea.
2013.10.31
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Ultra High Speed Nanomaterial Synthesis Process Developed Using Laser
Dr. Jun-Yeop, Yeo and the research team led by Professor Seung-Hwan, Ko (both of the Department of Mechanical Engineering) successfully developed a process enabling the location-determinable, ultra high speed synthesis of nanomaterials using concentrated laser beams. The result of the research effort was published as the frontispiece in the July 9th issue of Advanced Functional Materials, a world renowned material science and engineering academic journal. Application of the technology reduced the time needed to process nanomaterial synthesis from a few hours to a mere five minutes. In addition, unlike conventional nanomaterial synthesis processes, it is simple enough to enable mass production and commercialization. Conventional processes require the high temperatures of 900~1,000 °C and the use of toxic or explosive vapors. Complex processes such as separation after synthesis and patterning are needed for application in electronic devices. The multi-step, expensive, environmentally unfriendly characteristics of nanomaterial synthesis served as road blocks to its mass production and commercialization. Exposing the precursor to concentrated continuous laser beam (green wavelength) resulted in the synthesis of nanowires in the desired location; the first instance in the world to accomplish this feat. The technology, according to the research team, makes possible the production, integration and patterning of nanomaterials using a single process. Applicable to various surfaces and substrates, nanowires have been successfully synthesized on flexible plastic substrates and controlled patterning on the surface of 3-dimensional structures. Dr. Yeo commented that the research effort has “yielded the creation of a nanomaterial synthesis process capable of synthesis, integration, pattern, and material production using light energy” and has “reduced the synthesis process time of nanomaterial to one tenths of the conventional process.” Dr. Yeo continues to devise steps to commercialize the new multifunctional electronic material and methods for mass production. The research effort, led by Dr. Yeo and Professor Ko, received contribution from Professor Hyung-Jin Sung (KAIST Department of Mechanical Engineering), Seok-Joon Hong, a Ph.D. candidate, Hyun-Wook Kang, also a Ph.D. candidate, Professor Costas Grigoropoulos of UC Berkeley, and Dr. Dae Ho Lee. In addition, the team received support from the National Research Foundation, Ministry of Knowledge Economy, Global Frontier Program, and KAIST EEWS. Picture I: Synthesized nanomaterials produced at a desirable location by laser beams Picture 2: Synthesized nanomaterials built on the 3D structure by using the developed technology Picture 3: Functional electric circuit made with synthesized nanomaterials Picture 4: Cover page of July 9th issue of Advanced Functional Materials
2013.08.23
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KAIST unveils foldable micro electric car, Armadillo-T
The small and light electric car completely folds in half when parking, making it a perfect fit for public or private transportation in an urban environment. Looking for a parking space for hours at a busy shopping mall or being stuck on roads jammed with cars releasing large amounts of carbon dioxide are all-too-familiar scenes for city dwellers. A group of researchers at the Korea Advanced Institute of Science and Technology (KAIST) recently developed a possible solution to such problems: a foldable, compact electric vehicle that can be utilized either as a personal car or part of the public transit system to connect major transportation routes within a city. In-Soo Suh, associate professor of the Graduate School for Green Transportation at KAIST, and his research team introduced a prototype micro electric car called "Armadillo-T," whose design is based on a native animal of South America, the armadillo, a placental mammal with a leathery armor shell. The research team imitated the animal"s distinctive protection characteristic of rolling up into a ball when facing with threat from predators. Just as armadillos hide themselves inside the shell, Armadillo-T tucks its rear body away, shrinking its original size of 2.8 meters (110 inches) down to almost half, 1.65 meters (65 inches), when folding. Armadillo-T is a four-wheel-drive, all-electric car with two seats and four in-wheel motors. Since the motors are installed inside the wheels, and the 13.6 kWh capacity of lithium-ion battery pack is housed on the front side, the battery and motors do not have to change their positions when the car folds. This not only optimizes the energy efficiency but also provides stability and ample room to drivers and passengers. Once folded, the small and light (weighs 450 kg) electric vehicle takes up only one-third of a 5-meter parking space, the standard parking size in Korea, allowing three of its kind to be parked. With a smartphone-interfaced remote control on the wheels, the vehicle can turn 360 degrees, enhancing drivers" convenience to park the car, even in an odd space in a parking lot, the corner of a building, for example. Professor In-Soo Suh said, "I expect that people living in cities will eventually shift their preferences from bulky, petro-engine cars to smaller and lighter electric cars. Armadillo-T can be one of the alternatives city drivers can opt for. Particularly, this car is ideal for urban travels, including car-sharing and transit transfer, to offer major transportation links in a city. In addition to the urban application, local near-distance travels such as tourist zones or large buildings can be another example of application." The concept car has loads of smart features on board, too: the cameras installed inside the car eliminate the need for side mirrors and increase the driver"s ability to see the car"s right and left side, thereby reducing blind spots. With a smartphone, the driver can control Armadillo-T and enable remote folding control. The car has a maximum speed of 60 km/h, and with a ten-minute fast charge, it can run up to 100 km. Professor Suh explained that the concept of Armadillo-T was originally initiated in 2011 as he focused his research interest on the sub-A segment of personal mobility vehicles (PMVs), which are smaller and lighter than the current compact cars, as a new personalized transport mode. "In coming years, we will see more mega-size cities established and face more serious environmental problems. Throughout the world, the aging population is rapidly growing as well. To cope with climate, energy, and limited petroleum resources, we really need to think outside the box, once again, to find more convenient and eco-friendly transportation, just as the Ford Model T did in the early 1920s. A further level of R&D, technical standards, and regulatory reviews are required to have these types of micro vehicles or PMVs on the market through test-bed evaluations, but we believe that Armadillo-T is an icon toward the future transport system with technology innovation." The research project has been supported by the Korean government, the Ministry of Land, Infrastructure and Transport and the Korea Agency for Infrastructure Technology Advancement, since December 2012.Youtube Link: http://www.youtube.com/watch?v=8DoZH7Y-sR0
2013.08.21
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High Speed Nanomanufacturing Process Developed using Laser
Dr. Yeo Jun Yeop from KAIST’s Department of Mechanical Engineering, in a joint research project with Prof. Seung Hwan Ko, has developed a technology that speeds up the nanomanufacturing process by using lasers. Their research is published in the frontispiece of Advanced Functional Materials (July 9th issue). Fig. The frontispiece of Advanced Functional Materials(July 9th issue) The research group put a nanomaterial precursor on the board, illuminated it with a continuous-wave laser in the green wavelength range, and succeeded in synthesizing a nanowire at the point they wanted for the first time in the world. Currently nanomaterials are difficult to mass produce and commercialize due to their complex and costly manufacturing processes which also use toxic gases. However, their new technology simplified the process and so reduced the manufacturing time from some hours to five minutes (1/10th times reduced). Furthermore, this technology will apply regardless of the type of the board. Such nanometerials can be synthesized at any point on a flexible plastic board or even in three dimensional structures by illuminating them with a simple laser. Academics and industries expect mass production and commercialization of nanomaterials in near future. Dr. Yeo said he intends to research further to promote early commercialization of multifunctional electronic devices by combining various nanomaterials This research is sponsored by the National Research Foundation of Korea, the Ministry of Trade, Industry and Energy and KAIST EEWS Fig. A nanomaterial synthesized after illuminated by lasers Fig. A nanomaterial synthesized on a three dimensional structure using the developed technology Fig. Functional electron device manufactured by using the synthesized nanomaterials
2013.08.02
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A magnetic pen for smartphones adds another level of conveniences
Utilizing existing features on smartphones, the MagPen provides users with a compatible and simple input tool regardless of the type of phones they are using. A doctoral candidate at the Korea Advanced Institute of Science and Technology (KAIST) developed a magnetically driven pen interface that works both on and around mobile devices. This interface, called the MagPen, can be used for any type of smartphones and tablet computers so long as they have magnetometers embedded in. Advised by Professor Kwang-yun Wohn of the Graduate School of Culture Technology (GSCT) at KAIST, Sungjae Hwang, a Ph.D. student, created the MagPen in collaboration with Myung-Wook Ahn, a master"s student at the GSCT of KAIST, and Andrea Bianchi, a professor at Sungkyunkwan University. Almost all mobile devices today provide location-based services, and magnetometers are incorporated in the integrated circuits of smartphones or tablet PCs, functioning as compasses. Taking advantage of built-in magnetometers, Hwang"s team came up with a technology that enabled an input tool for mobile devices such as a capacitive stylus pen to interact more sensitively and effectively with the devices" touch screen. Text and command entered by a stylus pen are expressed better on the screen of mobile devices than those done by human fingers. The MagPen utilizes magnetometers equipped with smartphones, thus there is no need to build an additional sensing panel for a touchscreen as well as circuits, communication modules, or batteries for the pen. With an application installed on smartphones, it senses and analyzes the magnetic field produced by a permanent magnet embedded in a standard capacitive stylus pen. Sungjae Hwang said, "Our technology is eco-friendly and very affordable because we are able to improve the expressiveness of the stylus pen without requiring additional hardware beyond those already installed on the current mobile devices. The technology allows smartphone users to enjoy added convenience while no wastes generated." The MagPen detects the direction at which a stylus pen is pointing; selects colors by dragging the pen across smartphone bezel; identifies pens with different magnetic properties; recognizes pen-spinning gestures; and estimates the finger pressure applied to the pen. Notably, with its spinning motion, the MagPen expands the scope of input gestures recognized by a stylus pen beyond its existing vocabularies of gestures and techniques such as titling, hovering, and varying pressures. The tip of the pen switches from a pointer to an eraser and vice versa when spinning. Or, it can choose the thickness of the lines drawn on a screen by spinning. "It"s quite remarkable to see that the MagPen can understand spinning motion. It"s like the pen changes its living environment from two dimensions to three dimensions. This is the most creative characteristic of our technology," added Sungjae Hwang. Hwang"s initial research result was first presented at the International Conference on Intelligent User Interfaces organized by the Association for Computing Machinery and held on March 19-22 in Santa Monica, the US. In the next month of August, the research team will present a paper on the MagPen technology, entitled "MagPen: Magnetically Driven Pen Interaction On and Around Conventional Smartphones" and receive an Honorable Mention Award at the 15th International Conference on Human-Computer Interaction with Mobile Devices and Services (MobileHCI 2013) to be held in Germany. In addition to the MagPen, Hwang and his team are conducting other projects to develop different types of magnetic gadgets (collectively called "MagGetz") that include the Magnetic Marionette, a magnetic cover for a smartphone, which offers augmented interactions with the phone, as well as magnetic widgets such as buttons and toggle interface. Hwang has filed ten patents for the MagGetz technology. Youtube Links: http://www.youtube.com/watch?v=NkPo2las7wc, http://www.youtube.com/watch?v=J9GtgyzoZmM
2013.07.25
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Prof. Song Chong received the IEEE William R. Bennett Prize Paper Award
The IEEE (Institute of Electrical and Electronics Engineers) Communications Society (ComSoc), a renowned global network of professionals with a common interest in advancing communications technologies, has announced the winner of the 2013 William R. Bennett Prize in the field of communications networking. The prize was given to a Korean research team led by Song Chong, Professor of Electrical Engineering at KAIST and Injong Rhee, Professor of Computer Science at North Carolina State University. In addition, Dr. Minsu Shin, Dr. Seongik Hong, and Dr. Seong Joon Kim of Samsung Electronics Co., Ltd. as well as Professor Kyunghan Lee from Ulsan National Institute of Science and Technology were recognized for their contribution. The William R. Bennett Prize for communications networking has been awarded each year since 1994 in recognition of the best paper published in any journal financially sponsored or co-sponsored by ComSoc in the previous three calendar years. Only one paper per year is selected based on its quality, originality, scientific citation index, and peer reviews. Among the previous award winners are Robert Gallager of MIT, and Steven Low of the California Institute of Technology, and Kang G. Shin of the University of Michigan. The Korean research team’s paper, On the Levy-Walk Nature of Human Mobility, was published in the June 2011 issue of IEEE/ACM Transactions on Networking, a bimonthly journal co-sponsored by the IEEE ComSoc, the IEEE Computer Society, and the Association for Computing Machinery (ACM) with its Special Interest Group on Data Communications (SIGCOMM). In the paper, the research team proposed a new statistical model to effectively analyze the pattern of individual human mobility in daily life. The team handed out GPS (global positioning system) devices to 100 participants residing in five different university campuses in Korea and the US and collected data on their movements for 226 days. The mobility pattern obtained from the experiment predicted accurately how the participants actually moved around during their routines. Since publication, the paper has been cited by other papers approximately 350 times. The team’s research results will apply to many fields such as the prevention and control of epidemics, the design of efficient communications networks, and the development of urban and transportation system. The research team received the award on June 10th at the 2013 IEEE International Conference on Communications (ICC) held in Budapest, Hungary, from June 9-13, 2013. Professor Song Chong
2013.07.06
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Prof. Jong Chul Ye Appointed as the Editor of IEEE TIP
Professor Jong Chul Ye KAIST Bio & brain engineering department’s Professor Jong Chul Ye has been appointed as the editor of the "IEEE image processing transactions (IEEE TIP, IEEE Transaction on Image Processing)’, a prominent journal in the sector of imaging and medical image processing. Professor Ye will act as the editor in the field of medical imaging from February 2013 to January 2016, during which he will participate in examining thesis, establishing the direction of the journal and more. Professor Jong Chul Ye was recognized for his notable work in the field of medical imaging research using compressed sensing for the development of a high resolution medical image reconstruction techniques. This technique has pioneered a new area that is applicable in magnetic resonance imaging (MRI), computed tomography (CT), positron emission Camcorder (PET) and brain imaging. On the other hand, “IEEE TIP” was first published in 1992 and is currently the world’s leading authority in the field of image processing, medical imaging, image acquisition, compression and output.
2013.02.21
View 9455
New BioFactory Technique Developed using sRNAs
Professor Sang Yup Lee - published on the online edition of Nature Biotechnology. “Expected as a new strategy for the bio industry that may replace the chemical industry.”- KAIST Chemical & Biomolecular engineering department’s Professor Sang Yup Lee and his team has developed a new technology that utilizes the synthetic small regulatory RNAs (sRNAs) to implement the BioFactory in a larger scale with more effectiveness. * BioFactory: Microbial-based production system which creates the desired compound in mass by manipulating the genes of the cell. In order to solve the problems of modern society, such as environmental pollution caused by the exhaustion of fossil fuels and usage of petrochemical products, an eco-friendly and sustainable bio industry is on the rise. BioFactory development technology has especially attracted the attention world-wide, with its ability to produce bio-energy, pharmaceuticals, eco-friendly materials and more. For the development of an excellent BioFactory, selection for the gene that produces the desired compounds must be accompanied by finding the microorganism with high production efficiency; however, the previous research method had a complicated and time-consuming problem of having to manipulate the genes of the microorganism one by one. Professor Sang Yup Lee’s research team, including Dr. Dokyun Na and Dr. Seung Min Yoo, has produced the synthetic sRNAs and utilized it to overcome the technical limitations mentioned above. In particular, unlike the existing method, this technology using synthetic sRNAs exhibits no strain specificity which can dramatically shorten the experiment that used to take months to just a few days. The research team applied the synthetic small regulatory RNA technology to the production of the tyrosine*, which is used as the precursor of the medicinal compound, and cadaverine**, widely utilized in a variety of petrochemical products, and has succeeded developing BioFactory with the world’s highest yield rate (21.9g /L, 12.6g / L each). *tyrosine: amino acid known to control stress and improve concentration **cadaverine: base material used in many petrochemical products, such as polyurethane Professor Sang Yup Lee highlighted the significance of this research: “it is expected the synthetic small regulatory RNA technology will stimulate the BioFactory development and also serve as a catalyst which can make the chemical industry, currently represented by its petroleum energy, transform into bio industry.” The study was carried out with the support of Global Frontier Project (Intelligent Bio-Systems Design and Synthesis Research Unit (Chief Seon Chang Kim)) and the findings have been published on January 20th in the online edition of the worldwide journal Nature Biotechnology.
2013.02.21
View 9962
Professor Hwang Kyu Young and Professor Yang Dong Yeol Receives Engineer of Korea Award
Emeritus Professor Hwang Kyu Young (Department of Computer Sciences) and Professor Yang Dong Yeol (Department of Mechanical Engineering) were named as the 2012 Engineer of Korea by the Ministry of Education, Science, and Technology and Korea Science Foundation. The Engineer of Korea Award is awarded biannually to scientists and engineers that have contributed to the development of Korea’s science and technology and national economy. Professor Hwang’s work with DBMS and close coupling architecture of information search and overall new theories and application technology development in the field of database system has aided the opening and expansion of IT software industry development and the advent of internet information culture era. Professor Yang is a word renowned scholar in the field of net shape manufacturing and is considered to have opened a new page in the field of nano-molding technique. In addition, Professor Eum Sang Il (Department of Mathematical Science) has been selected as the 2012 Young Scientist Award.
2013.01.22
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KAIST Professors win 2012 Korea Engineering Award
Distinguished Professor Hwang Gyu Young (Department of Computer Science) and Professor Yang Dong Yol (Department of Mechanical Engineering) from KAIST received the 2012 ‘Korea Engineering Award’ hosted by the Ministry of Education, Science and Technology and the Korea Research Foundation. The ‘Korea Engineering Award’ is given biennially to researchers who have accomplished world class research and have contributed greatly to Korea’s development in the field of Science and Technology. The award started in 1994 and a total of 24 recipients were recognized in various fields such as electronics, mechanics, chemistry, construction, etc. The recipients of the award areawarded the Presidential award as well as 50million won as prize money. Professor Hwang was recognized for his research on DBMS close-coupling architecture as well as other new data base system theories, contributing to the development of the IT software industry in Korea. Professor Yang was praised for his work in precision shape creation and manufacturing, especially for his work in the nano-stereolithography process. In addition, Professor Oum Sang-il from the Deparment of Mathematical Science received the 2012 ‘Young Scientist Award’ hosted by the Ministry of Education, Science and Technology and the Korean Academy of Science and Technology. The ceremony for ‘Korea Engineering Award’ and the ‘Young Scientist Award’ was held in Seoul Press Center Press Club on the 21st of December.
2012.12.26
View 11749
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