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Prof. Song Chong received the IEEE William R. Bennett Prize Paper Award
The IEEE (Institute of Electrical and Electronics Engineers) Communications Society (ComSoc), a renowned global network of professionals with a common interest in advancing communications technologies, has announced the winner of the 2013 William R. Bennett Prize in the field of communications networking. The prize was given to a Korean research team led by Song Chong, Professor of Electrical Engineering at KAIST and Injong Rhee, Professor of Computer Science at North Carolina State University. In addition, Dr. Minsu Shin, Dr. Seongik Hong, and Dr. Seong Joon Kim of Samsung Electronics Co., Ltd. as well as Professor Kyunghan Lee from Ulsan National Institute of Science and Technology were recognized for their contribution.
The William R. Bennett Prize for communications networking has been awarded each year since 1994 in recognition of the best paper published in any journal financially sponsored or co-sponsored by ComSoc in the previous three calendar years. Only one paper per year is selected based on its quality, originality, scientific citation index, and peer reviews. Among the previous award winners are Robert Gallager of MIT, and Steven Low of the California Institute of Technology, and Kang G. Shin of the University of Michigan.
The Korean research team’s paper, On the Levy-Walk Nature of Human Mobility, was published in the June 2011 issue of IEEE/ACM Transactions on Networking, a bimonthly journal co-sponsored by the IEEE ComSoc, the IEEE Computer Society, and the Association for Computing Machinery (ACM) with its Special Interest Group on Data Communications (SIGCOMM).
In the paper, the research team proposed a new statistical model to effectively analyze the pattern of individual human mobility in daily life. The team handed out GPS (global positioning system) devices to 100 participants residing in five different university campuses in Korea and the US and collected data on their movements for 226 days. The mobility pattern obtained from the experiment predicted accurately how the participants actually moved around during their routines. Since publication, the paper has been cited by other papers approximately 350 times.
The team’s research results will apply to many fields such as the prevention and control of epidemics, the design of efficient communications networks, and the development of urban and transportation system.
The research team received the award on June 10th at the 2013 IEEE International Conference on Communications (ICC) held in Budapest, Hungary, from June 9-13, 2013.
Professor Song Chong
2013.07.06 View 12797 -
Foreign graduates donate to development fund for six years
International graduates of the Global IT Technology Graduate School at KAIST have continued a tradition of giving something back to their alma mater.
On May 10th, President Steve Kang held a donation ceremony with 12 donors.
Started in 2008, the graduates, mostly consisted of government officials from developing countries, have raised a development fund for KAIST, and in the past six years, a total of 81 graduates donated USD 10,000.
“KAIST provided me with an excellent education and research environment during my studies. I’m glad that I can have an opportunity to help my juniors and the university,” said Naryn Kenzhaliyev of Kazakhstan, a graduate present at the ceremony.
“Every year, these students voluntarily give when they are about to graduate. We feel proud and greatly appreciated for their philanthropic efforts,” said Professor Jae-Jung Noh, responsible for the global IT technology graduate program.
Established in 2006, the Global IT Technology Graduate School has attracted IT specialists from Korea and abroad, offering master’s and doctoral degrees. The school has invited many public servants in developing countries whose expertise lies in IT technology.
2013.07.04 View 6155 -
Thinking Out of the Box: KAIST Silicon Valley Innovation Platform
KAIST established a liaison office in San Jose, California, to support the entrepreneurship of KAIST graduates, students, and faculty who aspire to transform their innovative ideas into business.
The office, KAIST Silicon Valley Innovation Platform (SVIP), is located within the Korea Trade-Investment Promotion Agency (KOTRA) IT Center on North First Street in San Jose.
SVIP collects information and analyzes trends on emerging technologies; provides various educational programs on entrepreneurship and technology translation; offers opportunities to prospective entrepreneurs to engage with industry and research and government organizations; and assists Korean startups in accessing the US and North American market.
President Steve Kang attended the opening ceremony of the office on June 14th and encouraged KAIST alumni living in the US to share their ideas and technology innovations and transform them into business opportunities.
For more information, please contact Professor Soung-Hie Kim (seekim@business.kaist.ac.kr) from the Graduate School of Information and Media Management, KAIST.
2013.07.04 View 8396 -
KAIST Placed 3rd in the World's Best 100 Emerging Universities
The Times Higher Education (THE) published its world university rankings on June 20, 2013. It is a list of the best 100 universities whose histories are 50 years old or younger. KAIST was ranked 3rd in the list, two places up from the 5th last year. Forbes and Reuters carried a story on the top ten emerging universities out of the listed 100 institutions, highlighting strong showings in the Asian region. For the articles, please see the attached file (Forbes) or click the link (Reuters) below:
http://www.reuters.com/article/2013/06/19/education-university-rankings-idUSL2N0EU1HZ20130619
2013.06.22 View 7327 -
Nanofiber sensor detects diabetes or lung cancer faster and easier
Metal-oxide nanofiber based chemiresistive gas sensors offer greater usability for portable real-time breath tests that can be available on smart phones or tablet PCs in the near future.
Daejeon, Republic of Korea, June 11, 2013 -- Today"s technological innovation enables smartphone users to diagnose serious diseases such as diabetes or lung cancer quickly and effectively by simply breathing into a small gadget, a nanofiber breathing sensor, mounted on the phones.
Il-Doo Kim, Associate Professor of Materials Science and Engineering Department at the Korea Advanced Institute of Science and Technology (KAIST), and his research team have recently published a cover paper entitled "Thin-Wall Assembled SnO2 Fibers Functionalized by Catalytic Pt Nanoparticles and their Superior Exhaled Breath-Sensing Properties for the Diagnosis of Diabetes," in an academic journal, Advanced Functional Materials (May 20th issue), on the development of a highly sensitive exhaled breath sensor by using hierarchical SnO2 fibers that are assembled from wrinkled thin SnO2 nanotubes.
In the paper, the research team presented a morphological evolution of SnO2 fibers, called micro phase-separations, which takes place between polymers and other dissolved solutes when varying the flow rate of an electrospinning solution feed and applying a subsequent heat treatment afterward.
The morphological change results in nanofibers that are shaped like an open cylinder inside which thin-film SnO2 nanotubes are layered and then rolled up. A number of elongated pores ranging from 10 nanometers (nm) to 500 nm in length along the fiber direction were formed on the surface of the SnO2 fibers, allowing exhaled gas molecules to easily permeate the fibers. The inner and outer wall of SnO2 tubes is evenly coated with catalytic platinum (Pt) nanoparticles. According to the research team, highly porous SnO2 fibers, synthesized by eletrospinning at a high flow rate, showed five-fold higher acetone responses than that of the dense SnO2 nanofibers created under a low flow rate. The catalytic Pt coating shortened the fibers" gas response time dramatically as well.
The breath analysis for diabetes is largely based on an acetone breath test because acetone is one of the specific volatile organic compounds (VOC) produced in the human body to signal the onset of particular diseases. In other words, they are biomarkers to predict certain diseases such as acetone for diabetes, toluene for lung cancer, and ammonia for kidney malfunction. Breath analysis for medical evaluation has attracted much attention because it is less intrusive than conventional medical examination, as well as fast and convenient, and environmentally friendly, leaving almost no biohazard wastes.
Various gas-sensing techniques have been adopted to analyze VOCs including gas chromatography-mass spectroscopy (GC-MS), but these techniques are difficult to incorporate into portable real-time gas sensors because the testing equipment is bulky and expensive, and their operation is more complex. Metal-oxide based chemiresistive gas sensors, however, offer greater usability for portable real-time breath sensors.
Il-Doo Kim said, "Catalyst-loaded metal oxide nanofibers synthesized by electrospinning have a great potential for future exhaled breath sensor applications. From our research, we obtained the results that Pt-coated SnO2 fibers are able to identify promptly and accurately acetone or toluene even at very low concentration less than 100 parts per billion (ppb)."
The exhaled acetone level of diabetes patients exceeds 1.8 parts per million (ppm), which is two to six-fold higher than that (0.3-0.9 ppm) of healthy people. Therefore, a highly sensitive detection that responds to acetone below 1 ppm, in the presence of other exhaled gases as well as under the humid environment of human breath, is important for an accurate diagnosis of diabetes. In addition, Professor Kim said, "a trace concentration of toluene (30 ppb) in exhaled breath is regarded to be a distinctive early symptom of lung cancer, which we were able to detect with our prototype breath tester."
The research team has now been developing an array of breathing sensors using various catalysts and a number of semiconducting metal oxide fibers, which will offer patients a real-time easy diagnosis of diseases.
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Youtube Link: http://www.youtube.com/watch?v=t_Hr11dRryg
For further inquires:
Il-Doo Kim, Professor of Materials Science and Engineering, KAIST
Advanced Nanomaterials and Energy Laboratory
Tel: +82-42-350-3329
Email: idkim@kaist.ac.kr
Clockwise from left to right: left upper shows a magnified SEM image of a broken thin-wall assembled SnO2 fiber. Left below is an array of breath sensors (Inset is an actual size of a breath sensor). The right is the cover of Advanced Functional Materials (May 20th issue) in which a research paper on the development of a highly sensitive exhaled breath sensor by using SnO2 fibers is published.
This is the microstructural evolution of SnO2 nanofibers as a function of flow rate during electrospinning.
2013.06.20 View 13889
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Professor Jay H. Lee to receive the 2013 AIChE CAST Computing in Chemical Engineering Award
Professor Jay H. Lee of Chemical and Biomolecular Engineering Department at KAIST has won the 2013 Computing in Chemical Engineering Award of AIChE"s CAST Division (AIChE, American Institute of Chemical Engineers and CAST, Computing & Systems Technology Division).
The CAST Computing in Chemical Engineering Award, sponsored by The Dow Chemical Company, is annually given to an individual who has made outstanding contributions in the application of computing and systems technology to chemical engineering.Professor Lee has been recognized for his pioneering research contributions for “novel paradigms for much improved and robust model predictive control in industrial processes.” He is currently the Head of Chemical and Biomolecular Engineering Department and Director of Brain Korea (BK) 21 Program at the department. BK21 is the Korean government’s initiative to support the growth of research universities in the nation and foster highly trained master’s and doctoral students as well as researchers.
The CAST Computing in Chemical Engineering Award will be presented to Professor Jay H. Lee at the CAST Division dinner to be held at the AIChE Annual Meeting this November in San Francisco, where he will also deliver the after dinner lecture associated with this award.
2013.06.12 View 9885 -
KAIST Department of Mechanical Engineering Ranked in 19th Place
- Ranked in 19th place in 2013 Quacquarelli Symonds (QS) World University Rankings by Engineering, Mechanical, Aeronautical and Manufacturing Subjects -
KAIST ranked 19th in 2013 QS World University Rankings by Subject in Engineering, Mechanical, Aeronautical and Manufacturing Subjects. This is great progress compared to last year’s 51st-100th rank.
The 2013 QS World University Rankings used four indicators, including academic reputation, employer reputation, citations per paper, and H-index citations, to assess 2,858 universities in the world, and evaluated up to 200th place in 30 academic subjects.
KAIST earned high remarks from the H-index citations indicator, which is a new criteria introduced in the employer reputation rating. Moreover, the employer reputation section has risen sharply compared to the previous year. The H-index measures qualitatively and quantitatively the research outcomes of the researchers and assesses the number of papers written per professor and the average citation frequency of the papers.
The proportions of the indicators differ by subjects. For the mechanical engineering field, they weigh 40%, 30%, 15%, and 15%, respectively.
Rank
Academic
Employer
Citations per paper
H-index Citations
Score
19 (51-100)
68.1 (78.9)
89.1 (60.2)
84.6 (83.1)
93.1 (N/A)
80.4 (74.6)
2013.06.10 View 7389
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A KAIST research team developed in vivo flexible large scale integrated circuits
Daejeon, Republic of Korea, May 6th, 2013–-A team led by Professor Keon Jae Lee from the Department of Materials Science and Engineering at KAIST has developed in vivo silicon-based flexible large scale integrated circuits (LSI) for bio-medical wireless communication.
Silicon-based semiconductors have played significant roles in signal processing, nerve stimulation, memory storage, and wireless communication in implantable electronics. However, the rigid and bulky LSI chips have limited uses in in vivo devices due to incongruent contact with the curvilinear surfaces of human organs. Especially, artificial retinas recently approved by the Food and Drug Administration (refer to the press release of FDA"s artificial retina approval) require extremely flexible and slim LSI to incorporate it within the cramped area of the human eye.
Although several research teams have fabricated flexible integrated circuits (ICs, tens of interconnected transistors) on plastics, their inaccurate nano-scale alignment on plastics has restricted the demonstration of flexible nano-transistors and their large scale interconnection for in vivo LSI applications such as main process unit (MPU), high density memory and wireless communication. Professor Lee"s team previously demonstrated fully functional flexible memory using ultrathin silicon membranes (Nano Letters, Flexible Memristive Memory Array on Plastic Substrates), however, its integration level and transistor size (over micron scale) have limited functional applications for flexible consumer electronics.
Professor Keon Jae Lee"s team fabricated radio frequency integrated circuits (RFICs) interconnected with thousand nano-transistors on silicon wafer by state-of-the-art CMOS process, and then they removed the entire bottom substrate except top 100 nm active circuit layer by wet chemical etching. The flexible RF switches for wireless communication were monolithically encapsulated with biocompatible liquid crystal polymers (LCPs) for in vivo bio-medical applications. Finally, they implanted the LCP encapsulated RFICs into live rats to demonstrate the stable operation of flexible devices under in vivo circumstances.
Professor Lee said, "This work could provide an approach to flexible LSI for an ideal artificial retina system and other bio-medical devices. Moreover, the result represents an exciting technology with the strong potential to realize fully flexible consumer electronics such as application processor (AP) for mobile operating system, high-capacity memory, and wireless communication in the near future."
This result was published in the May online issue of the American Chemical Society"s journal, ACS Nano (In vivo Flexible RFICs Monolithically Encapsulated with LCP). They are currently engaged in commercializing efforts of roll-to-roll printing of flexible LSI on large area plastic substrates.
Movie at Youtube Link: Fabrication process for flexible LSI for flexible display, wearable computer and artificial retina for in vivo biomedical application
http://www.youtube.com/watch?v=5PpbM7m2PPs&feature=youtu.be
Applications of in Vivo Flexible Large Scale Integrated Circuits
Top: In vivo flexible large scale integrated circuits (LSI); Bottom: Schematic of roll-to-roll printing of flexible LSI on large area plastics.
2013.06.09 View 13071 -
International Student Conference (ICISTS-KAIST) to be Held in August
- 300 participants including university students worldwide and renowned speakers expected to gather
- Ideal coexistence of science & technology and society explored under the theme of “Perfect Alliance”
Science & technology and society are at the core of 21st century’s development. ICISTS-KAIST 2013, international conference for university students, seeks ways for the two to coexist harmoniously and is to be held from August 5 to 9 on KAIST campus as well as at Daejeon Convention Center.
ICISTS stands for International Conference for the Integration of Science, Technology and Society. ICISTS-KAIST is a non-profit organization run by KAIST students who are directly engaged in the coordination, planning, finance, public relations, and management of this academic event. The upcoming ninth annual event of ICISTS (www.icists.org) 2013 is centered around the theme, “Perfect Alliance: Coexistence for Human Society.” The conference will last for four nights and five days; scholars and students across various academic backgrounds gather to narrow the gap between fields of study and discuss possible solutions to the problems in today’s society.
The annual conference, ICISTS-KAIST attracts hundreds of participants from all over the world to KAIST, Daejeon and its most recent event last year witnessed discussions among some 300 students from 22 countries hearing the lectures from 40 academics and scholars. This year’s event will welcome the 16-year old inventor, scientist, and cancer researcher Jack Thomas Andraka, the founder of the “One Laptop Per Child” project Walter Bender, Chemistry Nobel Prize laureate Harold Walter Kroto, and many more.
The application period for ICISTS-KAIST 2013 runs from May 20 to July 12, and applications are received through the website at www.icists.org.
ICISTS-KAIST 2013 Promgram Summary
Event Title: International Conference for the Integration of Science, Technology and Society 2013 (ICISTS-KAIST 2013)
Theme: Perfect Alliance: Coexistence for Human Society
Date and Venue: 2013 Aug. 5 (Mon.) ~ Aug. 9 (Fri.), KAIST Campus and Daejeon Convention Center
Host and Organizer: ICISTS KAIST
Sponsor: Korean National Commission for UNESCO, Korea Tourism Organization, Korea Ministry of Education, Science & Technology, KOFST
Session Description:
Keynote Speech - Keynote address on fundamental approach to coexistence
Parallel Session - Multiple simultaneous lecture of delegates’ choice
Group Discussion - Small group discussions among delegates and speakers
Panel Discussion - In-depth and thought-revealing discussion among speakers
Experience Session - First-person experience on relevant technology
Team Project & Poster Fair - Team mission, poster exhibition and evaluation
Subtopics:
- New Values from Coexistence of Science & Technology and Society
- Synergetic Resolution via Coexistence of Science & Technology and Society
- Essential Communication for Coexistence of Science & Technology and Society
Notable Speakers:
- Gretchen Kalonji: Assistant to Director-General at UNESCO
- Sheila Jasanoff: Director of STS Program at Harvard Kennedy School
- Walter Bender: Former Director of MIT Media Lab and One Laptop Per Child- Jack Andraka: 16-year old Cancer Resesarcher
2013.05.31 View 8922 -
Neurotransmitter protein structure and operation principle identified
Professor Tae-Young Yoon
- Real-time measurement of structural change of bio-membrane fusion protein
- A new clue to degenerative brain diseases research
KAIST Physics Department’s Professor Tae-Young Yoon has successfully identified the hidden structure and operation mechanism of the SNARE protein, which has a central role in transporting neurotransmitters between neurons, using magnetic nanotweezers. SNARE protein’s cell membrane fusion function is closely related to degenerative brain diseases or neurological disorders such as Alzheimer’s. Hence, this research may provide a clue to the disease’s prevention and treatment.
Neurotransmission occurs when vesicles containing neurotransmitters fuse with cell membranes in neuron synapses. The SNARE protein is a cell-membrane fusion protein with a core role of releasing neurotransmitters. The academia speculated the SNARE protein would regulate the exchange of neurotransmitters, but its precise function and structure has been unknown. Professor Yoon’s research team developed an experimental technique using nanotweezers to measure physical changes to nanometer level by pulling and releasing each protein with force of 1 pN (piconewton). The research identified the existence of hidden SNARE protein"s intermediate structure. The process of withstanding and maintaining repulsive forces between bio-membranes in the hidden intermediate structure of SNARE to regulate the exchange of neurotransmitters has also been identified.
Professor Yoon’s research team developed an experimental technique using magnetic nanotweezers to measure physical changes of proteins to nanometer level by pulling and releasing each protein with force of 1 pN. The research identified the existence of hidden SNARE protein"s intermediate structure and its formation. The process of withstanding and maintaining repulsive forces between bio-membranes in the hidden intermediate structure of SNARE to regulate the exchange of neurotransmitters has also been discovered.
Professor Yoon said, “Ground breaking research results have been produced. A simple experimental technique of applying the smallest possible forces to proteins (with tweezers) to see their hidden structure and formation process can produce the same result as real observation has been developed.” He continued, “This technique will be very important in researching biological object with physical experimental technique. It will be a vital foundation to consilient research of different academia in the future.”
This research was a joint project of Physics Department’s Professor Tae-Young Yoon, KAIST, and Biomedical Engineering Institute’s Professor Yeon-Kyun Shin at KIST. KAIST Physics Department’s Professor Yong-Hoon Cho, Ph.D. candidate Do-Yong Lee and KIAS Computational Sciences Department’s Professor Chang-Bong Hyun participated. The research was published on Nature Communications on April 16th.
a) Neurotransmission occurs when vesicles containing neurotransmitters fuse with cell membranes in neuron synapses. A SNARE protein is a cell-membrane fusion protein with a core role of releasing neurotransmitters.
b) A schematic diagram using magnetic nanotweezers to measure protein structure changes on molecular level. The nanotweezers exert an exquisite pull and release of each protein with a force of 1 pN to measure physical changes to nanometer level in real-time to observe the hidden intermediate structure and operation principles of bio-membrane fusion protein.
2013.05.25 View 8972 -
KAIST Holds Robot Taekwondo Competition Recognized by the World Taekwondo Federation
KAIST will host the 12th Intelligent System-on-Chip (SoC) Robot War in October 2013, a robot competition. The event will have two entries: robot Taekwondo contest and HURO competition.
The World Taekwondo Federation has decided to offer an honorary Taekwondo degree to the winner of SoC Taekwondo Robot competition.
The Intelligent SoC Robot War was created in 2002 by KAIST’s Professor Hoi-Jun Yoo in the Department of Electrical Engineering.
For SoC Taekwondo Robot event, two robots compete in the form of Taekwondo, traditional Korean martial arts. The robots competing in this event have a camera and semiconductor chips on board, and therefore they have the brain-like functions to identify an object and control movements on their own. The robots have 21 joints with humanoid robot technology on their body for the techniques needed to compete in a typical Taekwondo match. They employ moves such as front kicks, side kicks, and upper punches.
In particular, KAIST’s System Design Innovation & Application Research Center, the organizer of this competition, has operated a team to demonstrate robot Taekwondo since last year with the purpose of displaying the basic movements of Taekwondo.
“Robots received attention as the source of growth in the near future. We have been developing robotics technology, and as part of our endeavor, preparing the Taekwondo demonstration team since 2012 to exhibit Korea’s robot technology and introduce our traditional martial arts,” said Professor Hoi-Jun Yoo. “We will continue to develop various capabilities for Taekwondo robots in cooperation with the World Taekwondo Federation.”
In HURO-Competition, robots compete for crossing the finishing line first by completing various missions, such as putting in a golf ball or overcoming obstacles while avoiding unexpected accidents. The winning team is awarded with a Presidential Award of Korea.
The 12th Intelligent SoC Robot War Competition is open to all graduate or undergraduate students. For details, visit the homepage at http://www.socrobotwar.org/.
2013.05.06 View 10382 -
The new era of personalized cancer diagnosis and treatment
Professor Tae-Young Yoon
- Succeeded in observing carcinogenic protein at the molecular level
- “Paved the way to customized cancer treatment through accurate analysis of carcinogenic protein”
The joint KAIST research team of Professor Tae Young Yoon of the Department of Physics and Professor Won Do Huh of the Department of Biological Sciences have developed the technology to monitor characteristics of carcinogenic protein in cancer tissue – for the first time in the world.
The technology makes it possible to analyse the mechanism of cancer development through a small amount of carcinogenic protein from a cancer patient. Therefore, a personalised approach to diagnosis and treatment using the knowledge of the specific mechanism of cancer development in the patient may be possible in the future.
Until recently, modern medicine could only speculate on the cause of cancer through statistics. Although developed countries, such as the United States, are known to use a large sequencing technology that analyses the patient’s DNA, identification of the interactions between proteins responsible for causing cancer remained an unanswered question for a long time in medicine.
Firstly, Professor Yoon’s research team has developed a fluorescent microscope that can observe even a single molecule. Then, the “Immunoprecipitation method”, a technology to extract a specific protein exploiting the high affinity between antigens and antibodies was developed. Using this technology and the microscope, “Real-Time Single Molecule co-Immunoprecipitation Method” was created. In this way, the team succeeded in observing the interactions between carcinogenic and other proteins at a molecular level, in real time.
To validate the developed technology, the team investigated Ras, a carcinogenic protein; its mutation statistically is known to cause around 30% of cancers.
The experimental results confirmed that 30-50% of Ras protein was expressed in mouse tumour and human cancer cells. In normal cells, less than 5% of Ras protein was expressed. Thus, the experiment showed that unusual increase in activation of Ras protein induces cancer.
The increase in the ratio of active Ras protein can be inferred from existing research data but the measurement of specific numerical data has never been done before.
The team suggested a new molecular level diagnosis technique of identifying the progress of cancer in patients through measuring the percentage of activated carcinogenic protein in cancer tissue.
Professor Yoon Tae-young said, “This newly developed technology does not require a separate procedure of protein expression or refining, hence the existing proteins in real biological tissues or cancer cells can be observed directly.” He also said, “Since carcinogenic protein can be analyzed accurately, it has opened up the path to customized cancer treatment in the future.”
“Since the observation is possible on a molecular level, the technology confers the advantage that researchers can carry out various examinations on a small sample of the cancer patient.” He added, “The clinical trial will start in December 2012 and in a few years customized cancer diagnosis and treatment will be possible.”
Meanwhile, the research has been published in Nature Communications (February 19). Many researchers from various fields have participated, regardless of the differences in their speciality, and successfully produced interdisciplinary research. Professor Tae Young Yoon of the Department of Physics and Professors Dae Sik Lim and Won Do Huh of Biological Sciences at KAIST, and Professor Chang Bong Hyun of Computational Science of KIAS contributed to developing the technique.
Figure 1: Schematic diagram of observed interactions at the molecular level in real time using fluorescent microscope. The carcinogenic protein from a mouse tumour is fixed on the microchip, and its molecular characteristics are observed live.
Figure 2: Molecular interaction data using a molecular level fluorescent microscope. A signal in the form of spike is shown when two proteins combine. This is monitored live using an Electron Multiplying Charge Coupled Device (EMCCD). It shows signal results in bright dots.
An organism has an immune system as a defence mechanism to foreign intruders. The immune system is activated when unwanted pathogens or foreign protein are in the body. Antibodies form in recognition of the specific antigen to protect itself. Organisms evolved to form antibodies with high specificity to a certain antigen. Antibodies only react to its complementary antigens. The field of molecular biology uses the affinity between antigens and antibodies to extract specific proteins; a technology called immunoprecipitation. Even in a mixture of many proteins, the protein sought can be extracted using antibodies. Thus immunoprecipitation is widely used to detect pathogens or to extract specific proteins.
Technology co-IP is a well-known example that uses immunoprecipitation. The research on interactions between proteins uses co-IP in general. The basis of fixing the antigen on the antibody to extract antigen protein is the same as immunoprecipitation. Then, researchers inject and observe its reaction with the partner protein to observe the interactions and precipitate the antibodies. If the reaction occurs, the partner protein will be found with the antibodies in the precipitations. If not, then the partner protein will not be found. This shows that the two proteins interact.
However, the traditional co-IP can be used to infer the interactions between the two proteins although the information of the dynamics on how the reaction occurs is lost. To overcome these shortcomings, the Real-Time Single Molecule co-IP Method enables observation on individual protein level in real time. Therefore, the significance of the new technique is in making observation of interactions more direct and quantitative.
Additional Figure 1: Comparison between Conventional co-IP and Real-Time Single Molecule co-IP
2013.04.01 View 18041