CT
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Success in Measuring Protein Interaction at the Molecular Level
Professor Tae Young Yoon
- Live observation of two protein interaction in molecular level successful- The limit in measurement and time resolution of immunoprecipitation technique improved by a hundred thousand fold
KAIST Department of Physics Professor Tae Young Yoon’s research team has successfully observed the interaction of two proteins live on molecular level and the findings were published in the October edition of Nature Protocols.
Professor Yoon’s research team developed a fluorescent microscope that can observe a single molecule. The team grafted the immunoprecipitation technique, traditionally used in protein interaction analysis, to the microscope to develop a “live molecular level immunoprecipitation technique”. The team successfully and accurately measured the reaction between two proteins by repeated momentary interactions in the unit of tens of milliseconds.
The existing immunoprecipitation technique required at least one day to detect interaction between two proteins. There were limitations in detecting momentary or weak interactions. Also, quantitative analysis of the results was difficult since the image was measured by protein-band strength. The technique could not be used for live observation.
The team aimed to drastically improve the existing technique and to develop accurate method of measurement on molecular level. The newly developed technology can enable observation of protein interaction within one hour. Also, the interaction can be measured live, thus the protein interaction phenomenon can be measured in depth.
Moreover, every programme used in the experiment was developed and distributed by the research team so source energy is secured and created the foundation for global infra.
Professor Tae Young Yoon said, “The newly developed technology does not require additional protein expression or purification. Hence, a very small sample of protein is enough to accurately analyse protein interaction on a kinetic level.” He continued, “Even cancerous protein from the tissue of a cancer patient can be analysed. Thus a platform for customised anti-cancer medicine in the future has been prepared, as well.”
Figure 1. Mimetic diagram comparing the existing immunoprecipitation technique and the newly developed live molecular level immunoprecipitation technique
2013.12.11 View 8445 -
KAIST Takes Steps towards a Self-Sustainable Campus
KAIST has been selected for a $45-million national smart grid initiative organized under the Ministry of Trade, Industry and Energy. Ninteen institutions will participate in the 2-year-long initiative. The consortium’s work is expected to take place from 2015 to 2017 after a review by the Ministry of Strategy and Finance.
The Smart Grid Explansion Initiative which has been considered the future of electric power industry implements information and communications technology to conventional grid system to maximize energy efficiency. The ROK government has selected the Smart Grid Expansion Initiative as one of South Korea’s primary national projects and plans to implement it nationwide based on multiple demonstration projects in major cities including Jeju.
KAIST plans to invest $45 million in developing systems for renewable energy power plants, efficient energy management, smart grid data, and electric vehicles to build the energy self-sustainable campus. It also hopes to contribute to fostering specialized talents and companies in energy management.
Byoung-Yoon Kim, the vice-president for research at KAIST, expects that by 2017, KAIST will be able to dramatically improve its energy capacity especially during peak periods and gain energy efficiency around the campus. He hopes that the micro grid project at KAIST will set a new standard for the self-sustainable campus.
2013.12.11 View 9329 -
Graduate Student at KAIST Awarded Best Prize at the 9th Inside Edge
Sun-Jin Choi, a Ph. D. candidate in the Department of Materials Science and Engineering at KAIST, under the guidance of Professor Il-Doo Kim, won the best prize at the 9th Inside Edge Contest hosted by Samsung Electro-Mechanics.
Choi was awarded prize money totaling fifteen million won at the award ceremony held on November 22 at the Mirae Hall at the headquarters of Samsung Electro-Mechanics in Suwon.
Choi’s research, titled “Exhaled Breath Sensor Arrays for the Non-invasive and Real-time Diagnosis of Diabetes by Detection of Acetone,” was recognized for its creativity and uniqueness.The Inside Edge is an international thesis competition which was started in 2005 to encourage and support creative research and potential technological development among young scientists and engineers.
Sun-Jin Choi (left) and Professor Il-Doo Kim (right).
2013.12.11 View 10200 -
Opening Ceremony of Genetic Donguibogam held
- Medicine using traditional natural substances • Food product source technology development begins
- Over 150,000,000,000 Won for 10 years of work invested to develop source technology
- Opening ceremony held on November 26th at 3 p.m. in Bio & Brain Engineering Division Building
The research to develop medicine and food source technology using traditional natural substances hasbegun.The opening ceremony of the “Genetic Donguibogam” business group, with KAIST Department of Bio & Brain Engineering Professor Do Heon Lee as the leader, was held on November 26th at 3 p.m. in Dream Hall, Bio & Brain Engineering Division Building, KAIST, Daejeon. The attendees of the opening ceremony included Yo Eop Im, Head of the Future Technology Department of the Ministry of Science, ICT and Future Planning and around 200 experts in science and technology industry, including the National Research Foundation of Korea, KAIST, the Korea Institute of Science and Technology, Seoul National University and Yonsei University.
The business group was established to re-interpret traditional natural substances proved to be effective from experience and improve quality of life by researching its applications; and to develop integrated source technology using traditional natural substances. The group is to invest over 150,000,000,000 Won for 10 years of research to secure natural substance source technology in five stages: interpretation technology, analysis technology, verification technology, bio marker technology and human body effectiveness verification technology. Especially, the focus would be on the use of virtual body computer models and Omics* to analyse the effects of traditional natural substances mixture on human body, and to find new materials for healthcare.
This research model, it is hoped, will have a new item to pioneer in the world natural substance market as well as securing a technologically competitive edge in bio industry by developing source technology that investigates the effects of traditional natural substances using cutting edge science.
KAIST Department of Bio & Brain Engineering Professor and Head Do Heon Lee of the “Genetic Donguibogam” Business Group said, “We will push forward to develop source energy by integrating IT-BT technology with a computer virtual body to build a cooperation system with medicine and functional food industries.” He continued: “This will enable not only the creation of a new industry, but also customised medicine.”
The 12 partners of the group include KAIST, Korea Institute of Science and Technology, Seoul National University and Yonsei University and 200 experts. The research participation area will be widened to foreign research institutes and associated companies.
* Terminology
Noun) Omics is an academic discipline analysing mass information on metabolism of physiological phenomena in specific cells (transcriptome, proteome and protoplast) with an integrated approach to determine vital phenomena.
2013.12.11 View 9876 -
Cambridge University Press and HISTAC to Publish Science and Civilization in Korea
The KAIST Research Institute for the History of Science, Technology and Civilization of Korea (HISTAC) and Cambridge University Press have agreed to publish a 10-volume collection entitled “Science and Civilization in Korea” in collaboration with the Needham Research Institute.
HISTAC was found in December 2012 with the support of the Academy of Korean Studies and the Korean Studies Promotion Service with the aim of publishing a collection composed of 30 Korean books and 7 English books on Korean science and civilization.
By November 2013, the HISTAC research team submitted a research paper composed of 11 Korean and 1 English book. It has now exceeded its initial goal of publishing 7 English books by signing the recent agreement with the Cambridge University Press.
“Science and Civilization in Korea” is the second collection of non-western science to be published by the Cambridge University Press since 1954 following “Science and Civilization in China” by Joseph Needham who is well-known for his momentous achievements in history of science in East Asia. This collection will highlight the achievements of Korea in science and civilization of Korea, much of which has been under-valued compared to those of China and Japan.[ It now has the significance similar to the Western science and civilization].
HISTAC appointed Professor Hong-Gi Yoon from the University of Auckland as the translator and invited Professor Christopher Cullen from Cambridge University and Professor Morris Low from the University of Queensland as co-editors. Professor Cullen was an editor of “Science and Civilization in China” and is now the director of the Needham Research Institute and Professor Low is an expert in modern science of East Asia.
The series includes:
- History of Science and Technology in Korea
- Technology, Everyday Life, and Korean Civilization
- History and Cultural Studies of Geomancy in Korea
- Patients, Doctors and the State: History of Korean Medical and Pharmaceutical Culture
- History of Astronomy in Korea
- Mathematics and the History of Korean Civilization
- The West and Korea in the History of Science and Technology, 1600-1950
- Imperialism, Colonialism, Post-colonialism and Technological Science in Korea
- Development of Science and Technology Under the Korean Authoritarian Regime
- Dynamics of Technological Development in Korean Industrialization
The HISTAC team believes that the publication will illuminate the nation’s triumphs in science and technology and expects that the publication will serve as valuable research resources for the study of the history of East Asian scientific civilization which has mainly focused on China and Japan. Further, by adopting various case studies of scientific achievements of South Korea and developing countries, they hope to propose a new model for studying history of science and civilization.
2013.11.28 View 9462
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President of WIPA Sang-Hui Lee and SK Hynix Awarded the 2013 Intellectual Property Award
The ceremony for the 3rd Intellectual Property Award was held at the KAIST campus in Seoul on November 2nd. The award is given annually to Korean practitioners in intellectual property.
This year’s recipients were Sang-Hui Lee, the president of the World Intellectual Property Association of Korean Practitioners (WIPA) and the SK Hynix Patent Group which successfully defended a long-lasting claim against a patent specialist corporation.
The Intellectual Property Award (IP Award) is presented in recognition to Korean individuals or groups that contributed to national competitiveness through creation, application, or proceedings of intellectual property, as well as to building the foundation for the protection of patents, intellectual properties, and trademarks.
President Lee successfully hosted the Global Intellectual Property Summit in Seoul, which was held in October 2012. He was a crucial force behind the foundation of WIPA in May of this year and was also elected as the first president for the organization. Recently, President Lee has been at the forefront of job creation through the efficient use of intellectual property and the creative discovery of venture endeavors.
President Lee said, “The unique characteristic of a knowledge-based society lies in the collaboration network of people and knowledge property, startups based on intellectual property, and decentralized development.”
He added, “Just as Finland adopted a policy to build venture companies in utilization of intellectual properties and encouraged the decentralized growth throughout the nation after the collapse of Nokia, Korea must adapt to meet the changing requirements of the knowledge-intensive era.”
The SK Hynix Patent Group, another recipient for the Intellectual Property Award, won the lawsuit, dragged on for 13 years, against the Rambus, an American patent specialized corporation. The group initially lost the first trial in the federal court of California, but through relentless research and efforts, they won the case in the appeals court. The IP Award was co-hosted by the Korea Patent Attorney Association, the Korea Intellectual Property and Service Association, and KAIST.
2013.11.21 View 9486 -
Exhibition of High Technology Held by the Graduate School of Culture Technology at KAIST
Screen X technology, displaying objects simutaneously on three screens
The Graduate School of Culture Technology at KAIST hosted the “2013 Demo Day” on the 1st of November.
The purpose of the Demo Day was to introduce recent research accomplishments on culture technology (CT) to the public and discuss the direction of the future trend of CT.The technologies introduced at the exhibition were: Screen X which simultaneously uses three screens, MagGetz which uses magnets for smart phone applications, Space Touch which connects the virtual reality with the real world, and Avatar that recognizes augmented objects using augmented reality.
Research papers entitled “Digital Art and Entertainment” (Professor Sung-Hee Lee), “Social Network” (Professor Mi-Young Cha), and “Interactive Media and Space” (Professor Ji-Hyun Lee) were presented as well. The Graduate School of Culture Technology was founded in 2005 to combine digital media and culture. Research is conducted largely in the fields of digital art & entertainment, ambient communication, and interactive media & space.
2013.11.20 View 6689 -
Professor Chun-Taek Rim Appointed as Associate Editor for IEEE TPEL
Professor Chun-Taek Rim of the nuclear and quantum engineering at KAIST was appointed as an associate editor of the Institute of Electrical and Electronics Engineers (IEEE) Transactions on Power Electronics (TPEL), an eminent academic journal bio-monthly published in the field of power electronics.The journal has a high impact factor (4.08), a measure reflecting the average number of citations to recent articles published in an academic journal, which ranks as the 6th the most influential journal among the 100 journals published by IEEE.Professor Rim was also appointed to an associate editor for IEEE Journal of Emerging and Selected Topics in Power Electronics in September in recognition of his expertise in wireless power and electric vehicles.
2013.11.15 View 11322 -
KAIST student wins Aerospace Student Papers Grand Prize
Dong-Il Yoo, a doctoral candidate under Professor Hyun-Chul Shim, at the Department of Aerospace Engineering, KAIST, has been awarded the Second Prize Award at the 11th Korea Aerospace Industries (KAI) Paper Contest. The award ceremony was held on October 30th at the media conference room at the KINTEX ADEX 2013 Exhibition in Seoul.
Yoo"s paper, titled "A Study on Virtual Pursuit Point-based Autonomous Air Combat Guidance Law for UCAV," is highly regarded for originality and creativity. The Field Robotics Center at the KAIST Institute, where Yoo conducted his research, also received the first prize at the 7th KAI Paper Contest.
The KAI Paper Contest was first organized in 2003 to promote academic interest and advance research and development in aerospace engineering among university students.
The KAI Paper Contest is one of the most prestigious contests in Korea. It is sponsored by the Ministry of Trade, Industry and Energy, the Ministry of Land, Infrastructure and Transport, the Korean Society for Aeronautical and Space Sciences, the Korea Aerospace Industries Association, and the Korea Civil Aviation Development Association.
Dong-Il Yoo (left) and Professor Hyun-Chul Shim (right)
2013.11.11 View 13249 -
KAIST Hosted the 6th International Presidential Forum on Global Research Universities
More than 120 global leaders from higher education, private and public sectors, to discuss the promotion of economic growth through knowledge creation and entrepreneurship
The Korea Advanced Institute of Science and Technology (KAIST) held the 6th International Presidential Forum on Global Research Universities (IPFGRU) on October 15th at the Westin Chosun Hotel in Seoul, Republic of Korea.
About 64 presidents and vice presidents from 57 research universities in 28 nations attended for a presentation and panel discussion on the topic of “The Role and Responsibility of Research Universities: Knowledge Creation, Technology Transfer, and Entrepreneurship.”Annually held, the forum is organized to promote excellence and innovation in higher education and provide a place for discussion among prominent research university leaders and key policy-makers in the private and public sectors from across the world.Among the notable universities attending the 2013 forum were the University of California, Irvine, the École Polytechnique Fédérale de Lausanne, Technische Universität Berlin, Technion-Israel Institute of Technology, Tokyo Institute of Technology, Rice University, the University of Waterloo, and Massachusetts Institute of Technology (MIT). Government officials as well as representatives from business and industry such as Samsung Electronics, Korea Telecom, and Elsevier also joined the event.
The forum was proceeded with three separate sessions: Enabling Knowledge Creation, Entrepreneurship & University-Based Technology Transfer, and Higher Education & Strategic Knowledge Creation: Specialization & Performance, through which speakers and panelists examined how universities have played a role in knowledge creation and technology transfer, and ultimately how they have contributed to the development of national economies.
Keynote speakers were Michael Drake, chancellor of UC Irvine, and Jörg Steinbach, president of Technische Universität Berlin. Forum participants shared their experiences and insights in starting up knowledge- and technolgy-based new businesses.
Steve Kang, president of KAIST, talked about the purpose of the 2013 IPFGRU:
“In the face of an ever-changing economic climate driven by shifts in technological advancement, demographic trends, and global integration, the role of research universities is becoming ever more significant in achieving sustainable economic growth. This forum will help participants from around the world to define the choices ahead as universities seek the most productive and beneficial models for cooperation with industry, venture startups, and government.”For the 2013 IPFGRU, Ministry of Science, ICT, and Future Planning, ROK, Saudi Aramco, Samsung Heavy Industries, S-Oil, Elsevier, Thomson Reuters, and the Korea Economic Daily were forum sponsors.
2013.11.04 View 11110 -
Collaboration with Korea Institute of Energy Research
KAIST and the Korea Institute of Energy Research (KIER) agreed on September 4th to further collaboration on energy research such as the development of nano-based hybrid solar cells, bio-fuels, artificial photosynthesis, and carbon dioxide reduction.
The two institutions will select 11 research projects to focus on their cooperation. President Steve Kang (in the right) stood with Jooho Whang, the president of KIER (in the left), holding the signed memorandum of understanding.
2013.11.04 View 8480 -
A powerful strategy for developing microbial cell factories by employing synthetic small RNAs
The current systems for the production of chemicals, fuels and materials heavily rely on the use of fossil resources. Due to the increasing concerns on climate change and other environmental problems, however, there has been much interest in developing biorefineries for the production of such chemicals, fuels and materials from renewable resources. For the biorefineries to be competitive with the traditional fossil resource-based refineries, development of high performance microorganisms is the most important as it will affect the overall economics of the process most significantly. Metabolic engineering, which can be defined as purposeful modification of cellular metabolic and regulatory networks with an aim to improve the production of a desired product, has been successfully employed to improve the performance of the cell. However, it is not trivial to engineer the cellular metabolism and regulatory circuits in the cell due to their high complexity.
In metabolic engineering, it is important to find the genes that need to be amplified and attenuated in order to increase the product formation rate while minimizing the production of undesirable byproducts. Gene knock-out experiments are often performed to delete those metabolic fluxes that will consequently result in the increase of the desired product formation. However, gene knock-out experiments require much effort and time to perform, and are difficult to do for a large number of genes. Furthermore, the gene knock-out experiments performed in one strain cannot be transferred to another organism and thus the whole experimental process has to be repeated. This is a big problem in developing a high performance microbial cell factory because it is required to find the best platform strain among many different strains. Therefore, researchers have been eager to develop a strategy that allows rapid identification of multiple genes to be attenuated in multiple strains at the same time.
A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) reported the development of a strategy for efficiently developing microbial cell factories by employing synthetic small RNAs (sRNAs). They first reported the development of such system in Nature Biotechnology last February. This strategy of employing synthetic sRNAs in metabolic engineering has been receiving great interest worldwide as it allows easy, rapid, high-throughput, tunable, and un-doable knock-down of multiple genes in multiple strains at the same time. The research team published a paper online on August 8 as a cover page (September issue) in Nature Protocols, describing the detailed strategy and protocol to employ synthetic sRNAs for metabolic engineering.
In this paper, researchers described the detailed step-by-step protocol for synthetic sRNA-based gene expression control, including the sRNA design principles. Tailor-made synthetic sRNAs can be easily manipulated by using conventional gene cloning method. The use of synthetic sRNAs for gene expression regulation provides several advantages such as portability, conditionality, and tunability in high-throughput experiments. Plasmid-based synthetic sRNA expression system does not leave any scar on the chromosome, and can be easily transferred to many other host strains to be examined. Thus, the construction of libraries and examination of different host strains are much easier than the conventional hard-coded gene manipulation systems. Also, the expression of genes can be conditionally repressed by controlling the production of synthetic sRNAs. Synthetic sRNAs possessing different repression efficiencies make it possible to finely tune the gene expression levels as well. Furthermore, synthetic sRNAs allow knock-down of the expression of essential genes, which was not possible by conventional gene knock-out experiments.
Synthetic sRNAs can be utilized for diverse experiments where gene expression regulation is needed. One of promising applications is high-throughput screening of the target genes to be manipulated and multiple strains simultaneously to enhance the production of chemicals and materials of interest. Such simultaneous optimization of gene targets and strains has been one of the big challenges in metabolic engineering. Another application is to fine tune the expression of the screened genes for flux optimization, which would enhance chemical production further by balancing the flux between biomass formation and target chemical production. Synthetic sRNAs can also be applied to finely regulating genetic interactions in a circuit or network, which is essential in synthetic biology. Once a sRNA scaffold-harboring plasmid is constructed, tailor-made, synthetic sRNAs can be made within 3-4 days, followed by the desired application experiments.
Dr. Eytan Zlotorynski, an editor at Nature Protocols, said "This paper describes the detailed protocol for the design and applications of synthetic sRNA. The method, which has many advantages, is likely to become common practice, and prove useful for metabolic engineering and synthetic biology studies."
This paper published in Nature Protocols will be useful for all researchers in academia and industry who are interested in the use of synthetic sRNAs for fundamental and applied biological and biotechnological studies.
This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) and the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea.
2013.10.31 View 10248