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Bio-based p-Xylene Oxidation into Terephthalic Acid by Engineered E.coli
KAIST researchers have established an efficient biocatalytic system to produce terephthalic acid (TPA) from p-xylene (pX). It will allow this industrially important bulk chemical to be made available in a more environmentally-friendly manner.
The research team developed metabolically engineered Escherichia coli (E.coli) to biologically transform pX into TPA, a chemical necessary in the manufacturing of polyethylene terephthalate (PET). This biocatalysis system represents a greener and more efficient alternative to the traditional chemical methods for TPA production. This research, headed by Distinguished Professor Sang Yup Lee, was published in Nature Communications on May 31.
The research team utilized a metabolic engineering and synthetic biology approach to develop a recombinant microorganism that can oxidize pX into TPA using microbial fermentation. TPA is a globally important chemical commodity for manufacturing PET. It can be applied to manufacture plastic bottles, clothing fibers, films, and many other products. Currently, TPA is produced from pX oxidation through an industrially well-known chemical process (with a typical TPA yield of over 95 mol%), which shows, however, such drawbacks as intensive energy requirements at high temperatures and pressure, usage of heavy metal catalysts, and the unavoidable byproduct formation of 4-carboxybenzaldehyde.
The research team designed and constructed a synthetic metabolic pathway by incorporating the upper xylene degradation pathway of Pseudomonas putida F1 and the lower p-toluene sulfonate pathway of Comamonas testosteroni T-2, which successfully produced TPA from pX in small-scale cultures, with the formation of p-toluate (pTA) as the major byproduct. The team further optimized the pathway gene expression levels by using a synthetic biology toolkit, which gave the final engineered E. coli strain showing increased TPA production and the complete elimination of the byproduct.
Using this best-performing strain, the team designed an elegant two-phase (aqueous/organic) fermentation system for TPA production on a larger scale, where pX was supplied in the organic phase. Through a number of optimization steps, the team ultimately achieved production of 13.3 g TPA from 8.8 g pX, which represented an extraordinary yield of 97 mol%.
The team has developed a microbial biotechnology application which is reportedly the first successful example of the bio-based production of TPA from pX by the microbial fermentation of engineered E. coli. This bio-based TPA technology presents several advantages such as ambient reaction temperature and pressure, no use of heavy metals or other toxic chemicals, the removable of byproduct formation, and it is 100% environmentally compatible.
Professor Lee said, “We presented promising biotechnology for producing large amounts of the commodity chemical TPA, which is used for PET manufacturing, through metabolically engineered gut bacterium. Our research is meaningful in that it demonstrates the feasibility of the biotechnological production of bulk chemicals, and if reproducible when up-scaled, it will represent a breakthrough in hydrocarbon bioconversions.”
Ph.D. candidate Zi Wei Luo is the first author of this research (DOI:10.1038/ncomms15689).The research was supported by the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT & Future Planning through the National Research Foundation of Korea.
Figure: Biotransformation of pX into TPA by engineered E. coli.
This schematic diagram shows the overall conceptualization of how metabolically engineered E. coli produced TPA from pX. The engineered E. coli was developed through reconstituting a synthetic metabolic pathway for pX conversion to TPA and optimized for increased TPA yield and byproduct elimination. Two-phase partitioning fermentation system was developed for demonstrating the feasibility of large-scale production of TPA from pX using the engineered E. coli strains, where pX was supplied in the organic phase and TPA was produced in the aqueous phase.
2017.06.05 View 10350 -
The 2017 International Food Festival
The savory smell of exotic dishes filled the main plaza of the KAIST campus on May 26. Exotic music reverberated throughout the campus. The KAIST community took a break together on a breezy early summer Friday afternoon sharing food with their friends and family. KAIST international student body, KISA (KAIST International Students Association), installed white food tents and prepared their country’s favorite dishes at their 13th annual International Food Festival.
The festival was co-organized with Chungnam National University and the University of Science & Technology (UST). At the festival, students from 18 nations cooked about 60 dishes and sold them to the public. Foreign students’ performances of traditional dance and music on the stage livened the atmosphere.
KISA President Sanzhar Kerimbek of the Department of Chemical and Biomolecular Engineering said, “We are so glad to show the diversity of KAIST and its rich culture. This is a big opportunity to get together with neighboring universities, CNU and UST and say thank you for their participation and support." Valentin Porcellini, an exchange students from France in the School of Computing, said, “We are so excited to have people taste our crepes, ratatouille, and other dishes.”
Associate Vice President of the International Office Jay Hyung Lee also said he was glad to see so many people joining this festival. While congratulating the students on the success of the festival, he said the festival will serve as an opportunity to better understand each other by sharing the food and culture.
(Photo caption: Paricipants stop by the Indonesian booth to purchase the food at the International Food Festival on May 26.)
2017.05.29 View 5113 -
Total Synthesis of Flueggenine C via an Accelerated Intermolecular Rauhut-Currier Reaction
The first total synthesis of dimeric securinega alkaloid (-)-flueggenine C was completed via an accelerated intermolecular Rauhut–Currier (RC) reaction. The research team led by Professor Sunkyu Han in the Department of Chemistry succeeded in synthesizing the natural product by reinventing the conventional RC reaction.
The total synthesis of natural products refers to the process of synthesizing secondary metabolites isolated from living organisms in the laboratory through a series of chemical reactions. Each stage of chemical reaction needs to be successful to produce the final target molecule, and thus the process requires high levels of patience and creativity. For that reason, the researchers working on natural products total synthesis are often called “molecular artists”.
Despite numerous reports on the total synthesis of monomeric securinegas, the synthesis of dimeric securinegas, whose monomeric units are connected by a putative enzymatic RC reaction, has not been reported to date.
The team used a Rauhut-Currier (RC) reaction, a carboncarbon bond forming a reaction between two Michael acceptors first reported by Rauhut and Currier in 1963, to successfully synthesize a dimeric natural product, flueggenine C. This new work featured the first application of an intermolecular RC reaction in total synthesis.
The conventional intermolecular RC reaction was driven non-selectively by a toxic nucleophilic catalyst at a high temperature of over 150°C and a highly concentrated reaction mixture, and thus has never been applied to natural products total synthesis. To overcome this long-standing problem, the research team placed a nucleophilic moiety at the γ-position of the enone derivative. As a result, the RC reaction could be induced by the simple addition of a base at ambient temperature and dilute solution, without the need of a nucleophilic catalyst. Using this newly discovered reactivity, the team successfully synthesized the natural product (-)-flueggenine C from commercially available amino acid derivative in 12 steps.
Professor Han said, “Our key finding regarding the remarkably improved reactivity and selectivity of the intermolecular RC reaction will serve as a significant stepping stone in allowing this reaction to be considered a practical and reliable chemical tool with broad applicability in natural products, pharmaceuticals, and materials syntheses. ”
This research was led by Ph.D. candidate Sangbin Jeon and was published in The Journal of the American Chemical Society (JACS) on May 10. This research was funded by KAIST start-up funds, HRHR (High-Risk High-Return), RED&B (Research, Education, Development & Business) projects, the National Research Foundation of Korea, and the Institute for Basic Science.
(Figure 1: Representative dimeric/oligomeric securinega alkaloids)
(Figure 2: Our reinvented Rauhut-Currier reaction)
(Figure 3: Total Synthesis of (-)-flueggenine C)
2017.05.23 View 8343 -
2017 KAIST Research Day Honors Professor Hoon Sohn
The 2017 KAIST Research Day recognized Professor Hoon Sohn of the Department of Civil and Environmental Engineering as Research Grand Prize Awardee in addition to the 10 most distinguished research achievements of the past year.
The Research Grand Prize recognizes the professor whose comprehensive research performance evaluation indicator is the highest over the past five years. The indicator combines the factors of the number of research contracts, IPR, royalty income, as well as research overhead cost inclusion.
During the ceremony, which was held on May 23, Professor Jun-Ho Oh of the Department of Mechanical Engineering and Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering also won the Best Research Award. The two professors had the best scores when evaluating their research performance for one-year periods.
Meanwhile, the Research Innovation Award went to Professor YongKeun Park of the Department of Physics. The Research Innovation Award scores the factors of foreign patent registration, contracts of technological transfer and income from technology fees, technology consultations, and startups and selected Professor Park as the top winner.
Professors Yong Hee Lee of the Department of Physics and Jonghwa Shin of the Department of Material Science won the Convergence Research Award. The Convergence Research Award recognizes the most outstanding research team who created innovative research results for a year.
After the ceremony, President Chen Shiyi of the Southern University of Science and Technology gave a distinguished lecture on the “Global & Entrepreneurial Universities for the Age of the Fourth Industrial Revolution.” the Research Day ceremony, KAIST also presented the ten most distinguished research achievements made by KAIST professors during the last year as follows (Click):
▲ Commercialization of 3D Holographic Microscopy by Professor YongKeun Park of the Department of Physics
▲ Designer Proteins with Chemical Modifications by Professor Hee-Sung Park of the Department of Chemistry
▲ Lanthanum-Catalyzed Synthesis of Microporous 3D Graphene-Like Carbons in a Zeolite Template by Professor Ryong Ryoo of the Department of Chemistry
▲ Complete Prevention of Blood Loss by Self-Sealing Hemostatic Needles by Professor Haeshin Lee of the Department of Chemistry
▲ An Immunological Mechanism for the Contribution of Commensal Microbiota Against Herpes Simplex Virus Infection in Genital Mucosa by Heung Kyu Lee of the Graduate School of Medical Science and Engineering
▲ Development of a Pulse-Echo Laser Ultrasonic Propagation Imaging System by Professor Jung-Ryul Lee of the Department of Aerospace Engineering
▲ Bi-refractive Stereo Imaging for Single-Shot Depth Acquisition by Professor Min H. Kim of the School of Computing
▲ Development of Environment Friendly Geotechnical Construction Material Using Biopolymer by Professor Gye-Chun Cho of the Department of Civil and Environmental Engineering
▲ Protein Delivery Via Engineered Exosomes by Professor Chulhee Choi of the Department of Bio and Brain Engineering
▲ Hot Electron Detection Under Catalytic Reactions by Professor Jeong Young Park of the Graduate School of EEWS.
After the ceremony, President Chen Shiyi of the Southern University of Science and Technology gave a distinguished lecture on the “Global & Entrepreneurial Universities for the Age of the Fourth Industrial Revolution.”
(Photo:President Shin poses with the 2017 KAIST Research Grand Prize Winner Professor Hoon Sohn on May 23.)
2017.05.23 View 11288 -
2017 ICISTS Conference 'Draw the Web: Interactions in Society'
The KAIST undergraduate organization, ICISTS (International Conference for Integration of Science, Technology and Society) will convene its annual conference from July 31 to Aug. 4 at the KAIST Daejeon Campus. This year’s theme is “Draw the Web: Interactions in Society.” More than 300 participants from 60 universities in 20 countries will participate in the international conference hosted and planned by the student organization.
Speakers at the 2017 conference include leaders in technology, business, investment, and entrepreneurship, and many others highlighted by Christoffer O. Hernæs, chief digital officer of Skandiabanken and vice president of strategy and innovation at Sparebank; Vincent C. Müller, professor of the philosophy division of humanities & social sciences at Anatolia College; Nigel Parker, director of developer and platform evangelism at Microsoft APAC; and Jon Gosier, founder and CEO of WoundedMetrics, who was voted as one of the 25 most influential African-Americans in technology by Business Insider in 2013 and 2014.
ICISTS has organized and hosted this event, the largest academic conference hosted and organized by students in Asia, since 2005 as a way to discuss an incredibly challenging issue: how science and technology is being integrated into society. This year’s conference will explore how prominent technological advancements are integrated, and how the interactions between humanity and technology will affect society. This year’s sub-theme is “Settlement, Movement, and Inequality.”
In addition to the main session, ICISTS is preparing discussion sessions in which guest speakers and participants will divide into small groups to discuss their responses to the themes. Various additional events including a culture night and an excursion program will serve as opportunities to network with other participants. For more information on the program and how to register, please visit http://www.icist.org.
2017.05.22 View 7095 -
Prof. Sang-Min Bae Receives 2017 iF Design Award
Prof. Sang-Min Bae and his research team from the Industrial Design Department of KAIST submitted a winning entry to the 2017 iF Design Award named ‘Culture BOXCHOOL’.
The iF Design Award is an internationally renowned design contest that is recognized as one of the top three design awards in the world along with the Red Dot Design Award and the IDEA Design Award. It has been held annually by iF International Forum Design since 1953. A total of 5,575 entries from 59 countries entered the last competition.
Culture BOXCHOOL is a modular container space platform designed for culture sharing in isolated areas. It is delivered as a standard shipping container along with its subsidiary modular parts and it transforms into a gallery, office, or classroom. These modular parts build the interior and exterior by attaching them to the corner castings, which are standard parts on all shipping containers. Two Cultural BOXCHOOL containers can be transformed into three different types of layouts.
The containers can generate their own energy using solar panels that provide sustainable energy to equipment inside. Additionally, hot humid air can flow out through the attic vent, doors, and windows.
“With Culture BOXCHOOL, you can easily and quickly create spaces such as offices and classrooms, or you can easily disassemble and move them to another location.
Thus, it can provide everyone with equal educational opportunities and cultural enjoyment regardless of their geographical location. In addition, because it produces its own energy, it is expected to create a cultural space in a relatively harsh environment such as in developing countries. These social and economic values of Culture BOXCHOOL seem to be what led to us winning the contest. I will continue to strive to create the world’s best designs for needy people.” Professor Bae said.
The ID+IM design laboratory, a research team led by Professor Bae, has been studying philanthropy design since 2005, working on solving various problems throughout society through innovative design. They have received more than 50 awards from the most prestigious design competitions in the world.
2017.05.18 View 6800 -
Distinguished Professor Lee Elected to the NAS
Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering was elected as a foreign associate to the US National Academy of Sciences (NAS) on May 2. The National Academy of Sciences elected 84 new members and 21 foreign associates in recognition of their distinguished and continuing achievements in their original research. Election to the Academy is widely regarded as one of the highest honors that a scientist can receive.
Professor Lee was also elected in 2010 as a member of the US National Academy of Engineering (NAE) for his leadership in microbial biotechnology and metabolic engineering, including the development of fermentation processes for biodegradable polymers and organic acids. Until 2016, there are only 12 people worldwide who are foreign associates of both NAS and NAE.
He is the first Korean elected to both prestigious academies, the NAS and the NAE in the US. Professor Lee is currently the dean of KAIST Institutes, the world leading institute for multi-and interdisciplinary research. He is also serving as co-chair of the Global Council on Biotechnology and member of the Global Future Council on the Fourth Industrial Revolution, the World Economic Forum.
2017.05.16 View 7876 -
Dr. Zi Jing Wong Named 2017 Jeong Hun Cho Awardee
(Photo caption: The 2017 Jeong Hun Cho Scholarship recipients pose with President Shin (left photo) and Dr. Zi Jing Wong, the recipient of the 2017 Jeong Hun Cho Award)
Dr. Zi Jing Wong, a postdoctoral scholar at the University of California, Berkeley was named the 2017 recipient of the Jeong Hun Cho Award. The award recognizes outstanding young scientists in the field of aerospace engineering annually. The recipient receives a 20 million KRW prize.
The Award Committee said that Dr. Wong who earned his MS at KAIST Department of Aerospace Engineering is a rising scholar in the fields of optic meta materials, photonics, imaging, among others. He has published five papers on the realization of a zero refractive index and the control of a refractive index, as well as the realization of a 3D invisibility cloak in Science and Nature Photonics in 2014 and 2015. Dr. Wong also swept the best paper awards from many international academic societies including the US Materials Research Society, IEEE, SPIE, and Metamaterials Congress in 2015. He finished his Ph.D. at the University of California, Berkeley.
The Award Committee also named three recipients of the Jeong Hun Cho Scholarship: Ph.D. candidate Hyon-Tak Kim of the Department of Aerospace Engineering at KAIST, Ph.D. candidate Ho-Song Park from the Department of Mechanical Engineering at Korea University, and Hyong-Jin Choi of Kongju National University High School.
The award was endowed by the family of the late Ph.D. candidate Jeong Hun Cho who died in a rocket lab accident in the Department of Aerospace Engineering in 2003. Cho was posthumously conferred an honorary doctorate degree.
In memory of Cho, his father established the ‘Jeong Hun Cho Award and Scholarship.’ The scholarship annually selects three young scholars from Cho’s alma maters of KAIST, Korea University, and Kongju National University High School.
2017.05.12 View 9773 -
Policy Debate Series for Industry 4.0
(Photo caption: President Shin takes the podium as the first speaker of a year-long monthly policy dabate series on Industry 4.0 on May 11.)
KAIST will kick off a monthly policy debate series on Industry 4.0 every Thursday from May 11 at the Startup KAIST building. The year-long series, featuring professors from key technology fields associated with Industry 4.0, is designed to help policy makers from government, industry, and research institutes respond better to the ramifications that Industry 4.0 brings about in each sector.
The series will help them establish the vision and strategy that will work for the new industrial environment to take the lead in the new industrial era.
Twelve professors, including President Sung-Chul Shin, from departments that are researching emerging technologies will speak on the megatrend of new technology, while facilitating debates and Q& A sessions with participants.
The participants will include officials from the government complexes in Sejong and Daejeon cities, government-funded research institutes in Daejeon, and businessmen, among others. For registration, please go to https://startup.kaist.ac.kr/register.
Schedule
Speaker
Theme
May 11
President Sung-Chul Shin
Challenges and Innovations of KAIST in the Era of Industry 4.0
June 8
Professor Jonghwan Kim
Machine Intelligence and Deep Learning
July 6
Professor Jun Ho Oh
Robot Technology and the Future
Aug. 3
Professor Hyunchul Shim
Unmanned Vehicle Technology and Industry 4.0
Sept. 7
Professor Hawoong Jeong
Complex Systems and Data Science
Oct. 12
Professor Yongdae Kim
Technology, Policy, and the Fostering of Talents: Industry 4.0 and Information Protection
Nov. 9
Professor Sang Yup Lee
The Role of Biotechnology in Industry 4.0
Dec. 7
Professor Meeyoung Cha
AI-Based Research for Fake News Detection
2018 Jan. 4
Professor Joungho Kim
Innovation for the Korean Semiconductor Industry: Kim’s Law
Feb. 8
Professor Jaekyun Moon
Education for Industry 4.0
March 8
Professor Sang Kil Cha
Artificial Intelligence Cyber Warfare: Its Present and Future
April 5
Professor Jaeseung Jeong
The Future of Brain Engineering and Artificial Intelligence
2017.05.08 View 8693 -
Professor Duck-Joo Lee Awarded the 21st Century Grand Prize
Professor Duck-Joo Lee of the Department of Aerospace Engineering was awarded the 21st Century Grand Prize in the field of technology development by the New Industry Management Academy and the 21st Leaders Club on April 13.
Professor Lee was honored in recognition of his contribution to the helicopter industry. He played a part in domestic helicopter development projects including the KUH-1 Surion (Korean Attack Helicopter), a twin-engine, transport utility helicopter as well as LAH (Light Armed Helicopter) and LCH (Light Civil Helicopter) projects.
Since joining KAIST in 1988, Professor Lee supervised more than 26 PhDs and 27 MSs. He was responsible for hosting the 1st Asian-Australian Rotorcraft Forum and Exhibition and currently serves as vice president of the American Helicopter Society and the Korea Drone Industry Promotion Association. He also participated in open online courses on K-MOOC and Coursera.
(Caption: Professor Lee (second from left in the first row) poses after receiving the award.)
2017.04.26 View 6476 -
Professor Jinah Park Received the Prime Minister's Award
Professor Jinah Park of the School of Computing received the Prime Minister’s Citation Ribbon on April 21 at a ceremony celebrating the Day of Science and ICT. The awardee was selected by the Ministry of Science, ICT and Future Planning and Korea Communications Commission.
Professor Park was recognized for her convergence R&D of a VR simulator for dental treatment with haptic feedback, in addition to her research on understanding 3D interaction behavior in VR environments. Her major academic contributions are in the field of medical imaging, where she developed a computational technique to analyze cardiac motion from tagging data.
Professor Park said she was very pleased to see her twenty-plus years of research on ways to converge computing into medical areas finally bear fruit. She also thanked her colleagues and students in her Computer Graphics and CGV Research Lab for working together to make this achievement possible.
2017.04.26 View 7878 -
FinTech Conference by KAIST, EDHEC-Risk Institute, Princeton, and Tsinghua
KAIST will partner with EDHEC-Risk Institute, Princeton University, and Tsinghua University to host a series of annual rotation conference on FinTech. The inaugural conference will be held in Princeton on April 26 and is entitled ‘Four-University Rotating FinTech Conference: Wealth Management Systems for Individual Investors.’
The conference will facilitate discussion among all interest parties of academics, practitioners, and regulators from around the world. Professor Woo Chang Kim of the Department of Industrial & Systems Engineering will represent KAIST. Professor Kim is also the head of the Center for Wealth Management Technologies at KAIST.
In addition to Professor Kim, leading experts from the US, Asia, and Europe will present at the conference, including Andrew Yao (Turing Award recipient and founder of IIIS FinTech Center at Tsinghua University), John Bogle (founder of the Vanguard Group, and president of the Bogle Financial Markets Research Center), Lionel Martellini (director of EDHEC-Risk Institute), John Mashey (Bell Labs/Silicon Valley computer scientist/corporate executive), and John Mulvey (professor and founding member of the Bendheim Center for Finance at Princeton University).
This year’s conference will feature following sessions:
· Mass-Customization of Goal-Based Investment Solutions: The New Frontier in Digital Wealth Management Services
· Goal-Based Investment via Multi-Stage Stochastic Goal Programming for Robo-Advisor Services
· Big Data – Yesterday, Today and Tomorrow
· Applying Machine Learning Concepts for Asset Allocation and ALM
· FinTech: Drawing Strengths from Computing Theories
· Savings and Investing to Achieve Retirement Goals: An Update Given Current Market Assumptions · The Rise of Robo-Advisors: A Threat or an Opportunity for the Wealth Management Industry?
The conference will include the participation of official partner Samsung Asset Management.
2017.04.20 View 7653