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New Diagnosis System for Cardiovascular Disease Developed
Professor Park Hyun Kyu of the department of Biological-Chemical Engineering developed a new diagnosis system for diagnosing cardiovascular diseases using E.coli to test the homocysteine concentration in the blood. The research team used the genetic recombination process to produce two different biologically illuminant nutrition cultures and compared the growth rate of the homocysteine between the two cultures by comparing the degree of luminescence. The technology can allow the simultaneous analysis of blood samples en masse and is also economical and thus is being regarded as a major step forward in the field of homocysteine concentration analysis which is a rapidly growing field. The conventional method used high performance liquid chromatography which took a long time to complete and was costly to run. The advantage of the newly developed system is that it gets rid of costly steps as it only needs to grow E.coli and measure the luminescence of the naturally occurring illuminant. The research was published as the cover paper of the April edition of ‘Analytical Chemistry’.
Low Cost and Simple Gene Analysis Technology Developed
Professor Park Hyun Kyu of the Department of Biology and Chemical Engineering has developed a ‘real time CPR’ using Methylene Blue (nucleic acid bonding molecule with Electro-Chemical property). The current gene analysis being used in the field is the real time PCR (Polymerase Chain Reaction) which takes advantage of the luminescent property of the gene and therefore requires expensive machines and chemicals to run. By contrast, the electro-chemical method is easy to use and low cost and, most importantly, it allows the machine to become small and portable. Professor Park’s research team used the decrease in the electro-chemical signal when the Methylene Blue reacts with nucleic acid and applied this to PCR which allowed for the real time analysis of the nucleic acid amplification process. With the result of the experiment as the basis, the team was able to perform a trial with Chlamydia trachomatis, a pathogen that causes sexually transmitted disease. The result showed that the electro-chemical method showed the same performance level as the real time PCR, which proved that the technology can be applied to diagnosing various diseases and gene research.
Explanation for the polymerized nucleic acid enzyme's abnormal activation found
KAIST’s Professor Park Hyun Kyu of the Department of Bio Chemical Engineering revealed on the 23rd of December 2010 that his team had successfully developed the technology that uses the metal ions to control the abnormal activation of nucleic acids’ enzymes and using this, created a logic gate, which is a core technology in the field of future bio electrons. The polymerized nucleic acid enzyme works to increase the synthesis of DNA and kicks into action only when the target DNA and primers form complimentary pairs (A and T, C and G). Professor Park broke the common conception and found that it is possible for none complimentary pairs like T-T and C-C to initiate the activation of the enzyme and thus increase the nucleic acid production, given that there are certain metal ions present. What Professor Park realized is that the enzymes mistake the uncomplimentary T-T and C-C pairs (with stabilized structures due to the bonding with mercury and silver ions) as being complimentary base pairs. Professor Park described this phenomenon as the “illusionary polymerase activity.” The research team developed a logic gate based on the “illusionary polymerase activity’ phenomenon.” The logic gate paves the way to the development of future bio electron needed for bio computers and high performance memories. Professor Park commented, “The research is an advancement of the previous research carried on about metal ions and nucleic acid synthesis. Our research is the first attempt at merging the concepts of the two previously separately carried out researches and can be adapted for testing presence of metal ions and development of a new single nucleotide polymorphic gene analysis technology.” Professor Park added that, “Our research is a great stride in the field of nano scale electron element research as the results made possible the formation of accurate logic gates through relatively cost efficient and simple system designs.” On a side note, the research was funded by Korea Research Foundation (Chairman: Park Chan Mo) and was selected as the cover paper for the December issue of ‘Angewandte Chemie International Edition’.
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