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Real+time+CPR%E2%80%99+using+Methylene+Blue+%28nucleic+acid+bonding+molecule+with+Electro-Chemical+property%29%0APCR+%28Polymerase+Chain+Reaction%29+Chlamydia+trachomatis+pathogen+diagnose

Low Cost and Simple Gene Analysis Technology Developed Professor Park Hyun Kyu of the Department of Biology and Chemical Engineering has developed a ‘real time CPR’ using Methylene Blue (nucleic acid bonding molecule with Electro-Chemical property). The current gene analysis being used in the field is the real time PCR (Polymerase Chain Reaction) which takes advantage of the luminescent property of the gene and therefore requires expensive machines and chemicals to run. By contrast, the electro-chemical method is easy to use and low cost and, most importantly, it allows the machine to become small and portable. Professor Park’s research team used the decrease in the electro-chemical signal when the Methylene Blue reacts with nucleic acid and applied this to PCR which allowed for the real time analysis of the nucleic acid amplification process. With the result of the experiment as the basis, the team was able to perform a trial with Chlamydia trachomatis, a pathogen that causes sexually transmitted disease. The result showed that the electro-chemical method showed the same performance level as the real time PCR, which proved that the technology can be applied to diagnosing various diseases and gene research.
2011.04.30 View 8211

KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea T.042-350-2114 F.042-350-2210(2220)